Microfluidic In-Flow Decantation Technique Using Stepped Pillar Arrays and Hydraulic Resistance Tuners
Microfluidic In-Flow Decantation Technique Using Stepped Pillar Arrays and Hydraulic Resistance Tuners
复制标题
使用阶梯柱阵列和液压阻力调谐器的微流体流入倾析技术
DOI：
10.3390/mi10070471
复制
发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
S. S. Gorthi
中科院分区：
文献类型：
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作者：
Gangadhar Eluru;P. Nagendra;S. S. Gorthi
从样品溶液的液体组分中分离颗粒对于几种基于微流体的样品制备和/或样品处理技术是重要的，例如从全血中分离血浆，无鞘流聚焦，颗粒富集等。本文提出了一种微流体流入倾析技术，该技术提供了从无颗粒流体中分离颗粒的同时流入。该设计涉及样品流体通道在横向和深度方向上的扩展，从而产生朝向通道壁的无颗粒层，随后通过朝向深度方向一端定位的一系列微小开口逐渐提取该无颗粒流体。该设计的后半部分在该倾析技术的功能中是非常关键的，并且基于称为wee提取的原理。介绍了设计、理论和模拟，以解释操作原理。为了证明原理验证，对珠粒、血小板和不同血细胞比容（2.5%-45%）的血液样本进行了实验表征。实验表明，在装置运行至少一小时内，无颗粒流体的无堵塞分离，并证明纯度接近100%，产率高达14%。提高产量的途径进行了讨论沿着与几个潜在的应用。
Separating the particles from the liquid component of sample solutions is important for several microfluidic-based sample preparations and/or sample handling techniques, such as plasma separation from whole blood, sheath-free flow focusing, particle enrichment etc. This paper presents a microfluidic in-flow decantation technique that provides the separation of particles from particle-free fluid while in-flow. The design involves the expansion of sample fluid channel in lateral and depth directions, thereby producing a particle-free layer towards the walls of the channel, followed by gradual extraction of this particle-free fluid through a series of tiny openings located towards one-end of the depth-direction. The latter part of this design is quite crucial in the functionality of this decantation technique and is based on the principle called wee-extraction. The design, theory, and simulations were presented to explain the principle-of-operation. To demonstrate the proof-of-principle, the experimental characterization was performed on beads, platelets, and blood samples at various hematocrits (2.5%–45%). The experiments revealed clog-free separation of particle-free fluid for at least an hour of operation of the device and demonstrated purities close to 100% and yields as high as 14%. The avenues to improve the yield are discussed along with several potential applications.
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DOI：
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发表时间：
2022
期刊：
影响因子：
--
作者：
早島理;長倉伯博;山本智佳央・平田修三・田邉哲雄・片山由季・中野紗樹
通讯作者：
山本智佳央・平田修三・田邉哲雄・片山由季・中野紗樹