Use of molecular assay in diagnosis of hand, foot and mouth disease caused by enterovirus 71 or coxsackievirus A 16
Use of molecular assay in diagnosis of hand, foot and mouth disease caused by enterovirus 71 or coxsackievirus A 16
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DOI：
10.1016/s0166-0934(01)00376-7
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发表时间：
2002-04-01
影响因子：
3.1
通讯作者：
Shih, SR
中科院分区：
文献类型：
--
作者：
Tsao, KC;Chang, PY;Shih, SR
手足口病是儿童常见病，通常由柯萨奇病毒A16和肠道病毒71型引起。与柯萨奇病毒A16相比，肠道病毒71型感染更为严重，严重并发症和病死率显著增加。因此，建立一种快速、特异的方法来区分手足口病暴发中的柯萨奇病毒A16和肠道病毒71型是非常重要的。在本研究中，我们设计了两套针对柯萨奇病毒A16和肠道病毒71型的RT-PCR引物。对189种病毒进行了分子诊断试验。在110株肠道病毒71型中，对107株临床分离的肠道病毒和3株肠道病毒71型参考株有清晰的信号。柯萨奇病毒A16、其他肠道病毒或非肠道病毒均未显示出对肠道病毒71型特异性引物的信号。另一方面，在28株柯萨奇病毒A16株中，柯萨奇病毒A16特异性引物检测到27株临床分离株和1株参考株，但与其他病毒没有交叉反应。本研究建立的分子检测方法为鉴别柯萨奇病毒A16和肠道病毒71型手足口病提供了一种敏感而特异的方法，为今后的暴发疫情提供了一种有用的快速诊断方法。(C)2002 Elsevier Science B.V.保留所有权利。
Hand, foot and mouth disease is a common illness in children and is usually caused by coxsackievirus A 16 and enterovirus 71. It has been noted that enterovirus 71 infection is more severe with significantly greater frequency of serious complications and fatality than coxsackievirus A 16. Therefore, it is important to develop a rapid and specific assay for discriminating coxsackievirus A 16 and enterovirus 71 in hand, foot and mouth disease outbreaks. In this study we designed two sets of RT-PCR primers specific for coxsackievirus A 16 and enterovirus 71. One hundred and eighty-nine viruses were evaluated for this molecular diagnosis assay. Among 110 enterovirus 71 strains, the enterovirus 71 specific primers gave clear signal for 107 clinical enterovirus 71 isolates and three reference enterovirus 71 strains. None of coxsackievirus A 16, other enteroviruses or non-enteroviruses show signal for enterovirus 71-specific primers. On the other hand, among 28 coxsackievirus A 16 strains, the coxsackievirus A 16-specific primers detect 27 clinical isolates and one reference strain but show no cross-reaction with other viruses. The molecular assay developed in this study provides a sensitive and specific way to distinguish coxsackievirus A 16 and enterovirus 71 induced hand, foot and mouth disease, which will be a useful rapid diagnostic method in future outbreaks. (C) 2002 Elsevier Science B.V. All rights reserved.