Studies on the origin of the precursor cells in multiple myeloma, Waldenström's macroglobulinaemia and benign monoclonal gammopathy. I. Cytoplasmic isotype and idiotype distribution in peripheral blood and bone marrow.
Studies on the origin of the precursor cells in multiple myeloma, Waldenström's macroglobulinaemia and benign monoclonal gammopathy. I. Cytoplasmic isotype and idiotype distribution in peripheral blood and bone marrow.
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多发性骨髓瘤、华氏巨球蛋白血症和良性单克隆丙种球蛋白病的前体细胞起源研究 I. 外周血和骨髓中的细胞质同型和独特型分布。
DOI：
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发表时间：
1981
影响因子：
4.6
通讯作者：
L. Broodtaerts
中科院分区：
文献类型：
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作者：
B. Van Camp;P. Reynaert;L. Broodtaerts
研究了多发性骨髓瘤、良性单克隆丙种球蛋白病和华氏巨球蛋白血症患者的外周血和骨髓中的淋巴细胞和浆细胞的细胞质免疫球蛋白分布。抗独特型血清用作单克隆性的标记。双波长荧光显微镜使得同时使用具有不同荧光染料的抗同种型和抗独特型血清成为可能。结论是，在骨髓中，单克隆事件从淋巴细胞水平开始，该淋巴细胞已经形成其最终同种型。骨髓中单克隆扩增的大小和参与增殖的细胞类型可能决定是否发生扩散。与骨髓中含有免疫球蛋白的细胞相比，外周血中含有细胞质免疫球蛋白的多克隆淋巴细胞减少，并且表现出相反的κ/λ比率。这一发现表明多克隆 B 细胞前体存在与轻链型相关的抑制。
Lymphocytes and plasma cells in the peripheral blood and bone marrow of patients with multiple myeloma, benign monoclonal gammopathy and Waldenström's macroglobulinaemia were investigated for their cytoplasmic immunoglobulin distribution. Anti-idiotypic sera were used as markers for monoclonality. Double-wavelength fluorescence microscopy made it possible simultaneously to use anti-isotype and anti-idiotype sera with different fluorochromes. It was concluded that, in the bone marrow, the monoclonal event starts at the level of a lymphoid cell which has already been committed to its final isotype. The size of the monoclonal expansion in the bone marrow and the cell types involved in the proliferation may determine whether spread occurs. Polyclonal lymphoid cells containing cytoplasmic immunoglobulins were decreased in the peripheral blood and exhibited a reversed kappa/lambda ratio when compared to the immunoglobulin-containing cells in the bone marrow. This finding suggests a light chain-type related depression of polyclonal B cell precursors.