Cell lineage in mammalian craniofacial mesenchyme
Cell lineage in mammalian craniofacial mesenchyme
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DOI：
10.1016/j.mod.2008.06.007
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发表时间：
2008-09-01
影响因子：
2.6
通讯作者：
Iseki, Sachiko
中科院分区：
文献类型：
--
作者：
Yoshida, Toshiyuki;Vivatbutsiri, Philaiporn;Iseki, Sachiko
我们分析了神经嵴和中胚层的哺乳动物颅面间充质及其衍生物的细胞谱系示踪实验在小鼠胚胎中的贡献，使用永久性遗传标记Wnt 1-cre的神经嵴和Mesp 1-cre的中胚层，结合Rosa 26报告。在神经嵴细胞迁移结束时（E9.5），这两种模式是相互的，在眼睛后面有一个共同的边界。中胚层细胞表达内皮标志物（angioblasts）被发现不尊重这一边界，他们与迁移的神经嵴从5体节阶段，并通过E9.5，他们形成一个前内皮网状整个颅间充质。肌源性谱系的中胚层细胞也随着神经嵴细胞迁移，如鳃弓的形成。到E17.5时，下外胚层间充质中的神经嵴-中胚层边界与位于额骨和顶骨之间的下骨骼生成层的边界不重合。在E13.5，这些骨骼的原基位于大脑的基底外侧，在接下来的4-5天内向头骨的顶部延伸。我们用DiI标记子宫外E13.5胚胎的骨原基，以区分额骨和顶骨起源的两种可能性：（1）从邻近结缔组织招募或（2）原始原基增殖。结果清楚地表明，骨原基通过内在生长垂直延伸，没有检测到相邻间充质细胞的募集。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
We have analysed the contributions of neural crest and mesoderm to mammalian craniofacial mesenchyme and its derivatives by cell lineage tracing experiments in mouse embryos, using the permanent genetic markers Wnt1-cre for neural crest and Mesp1-cre for mesoderm, combined with the Rosa26 reporter. At the end of neural crest cell migration (E9.5) the two patterns are reciprocal, with a mutual boundary just posterior to the eye. Mesodermal cells expressing endothelial markers (angioblasts) are found not to respect this boundary; they are associated with the migrating neural crest from the 5-somite stage, and by E9.5 they form a pre-endothelial meshwork throughout the cranial mesenchyme. Mesodermal cells of the myogenic lineage also migrate with neural crest cells, as the branchial arches form. By E17.5 the neural crest-mesoderm boundary in the subectodermal mesenchyme becomes out of register with that of the underlying skeletogenic layer, which is between the frontal and parietal bones. At E13.5 the primordia of these bones lie basolateral to the brain, extending towards the vertex of the skull during the following 4-5 days. We used DiI labelling of the bone primordia in ex-utero E13.5 embryos to distinguish between two possibilities for the origin of the frontal and parietal bones: (1) recruitment from adjacent connective tissue or (2) proliferation of the original primordia. The results clearly demonstrated that the bone primordia extend vertically by intrinsic growth, without detectable recruitment of adjacent mesenchymal cells. (C) 2008 Elsevier Ireland Ltd. All rights reserved.