Urinary phthalate metabolites and male reproductive function parameters in Chongqing general population, China
Urinary phthalate metabolites and male reproductive function parameters in Chongqing general population, China
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重庆市一般人群尿邻苯二甲酸酯代谢物与男性生殖功能参数
DOI：
10.1016/j.ijheh.2013.06.006
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发表时间：
2014-03-01
影响因子：
6
通讯作者：
Cao, Jia
中科院分区：
文献类型：
--
作者：
Han, Xue;Cui, Zhihong;Cao, Jia
本研究旨在调查中国重庆市一般人群邻苯二甲酸酯暴露水平，并确定邻苯二甲酸酯暴露与男性生殖功能参数之间的可能关系。通过重庆市计划生育研究所和重庆市生殖健康中心招募普通男性232例。在来自每个人的单点尿样中，使用固相萃取结合高效液相色谱和串联质谱检测来分析邻苯二甲酸酯代谢物，包括邻苯二甲酸单丁酯（MBP）、邻苯二甲酸单乙酯（MEP）、邻苯二甲酸单（2-乙基己基）酯（MEHP）、邻苯二甲酸单苄酯（MBzP）、邻苯二甲酸（PA）和总PA。精液参数根据世界卫生组织参考值进行二分。采用碱性单细胞凝胶电泳分析精子DNA损伤。用放射免疫法测定血清生殖激素水平。我们观察到重庆市普通人群尿MBP浓度与精子浓度之间的弱相关性。高于中位数的MBP水平是精子浓度低于参考值的1.97倍（95%置信区间[CI] 0.97-4.04）。在调整潜在风险因素后，邻苯二甲酸酯代谢物与生殖功能参数之间没有其他相关性。本研究提示，重庆地区一般人群暴露于邻苯二甲酸酯环境水平对生殖有微弱或无不良影响。(C)2013 Elsevier GmbH. All rights reserved.
This study was designed to investigate the phthalates exposure levels in general population in Chongqing City of China, and to determine the possible associations between phthalate exposure and male reproductive function parameters. We recruited 232 general men through Chongqing Family Planning Research Institute and Reproductive Center of Chongqing. In a single spot urine sample from each man, phthalate metabolites, including mono-butyl phthalate (MBP), mono-ethyl phthalate (MEP), mono-(2-ethylhexyl) phthalate (MEHP), mono-benzyl phthalate (MBzP), phthalic acid (PA), and total PA were analyzed using solid phase extraction and coupled with high-performance liquid chromatography and detection by tandem mass spectrometry. Semen parameters were dichotomized based on World Health Organization reference values. Sperm DNA damage were analyzed using the alkaline single-cell gel electrophoresis assay. Reproductive hormones were determined in serum by the radioimmunoassay kit. We observed a weak association between urinary MBP concentration and sperm concentration in Chongqing general population. MBP levels above the median were 1.97 times (95% confidence interval [CI] 0.97-4.04) more likely to have sperm concentration below the reference value. There were no other associations between phthalate metabolites and reproductive function parameters after adjusted for potential risk factors. Our study suggested that general population in Chongqing area of China exposure to the environmental level of phthalate have weak or without adverse effects on the reproduction. (C) 2013 Elsevier GmbH. All rights reserved.