Direct electrochemistry of horseradish peroxidase immobilized on DNA/electrodeposited zirconium dioxide modified, gold disk electrode
Direct electrochemistry of horseradish peroxidase immobilized on DNA/electrodeposited zirconium dioxide modified, gold disk electrode
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DOI：
10.1007/s10529-007-9319-4
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发表时间：
2007-02
影响因子：
2.7
通讯作者：
Zhongqiang Tong;R. Yuan;Y. Chai;Shihong Chen;Yi Xie
中科院分区：
文献类型：
--
作者：
Zhongqiang Tong;R. Yuan;Y. Chai;Shihong Chen;Yi Xie
报道了一种基于DNA/电沉积ZrO 2/修饰金电极固定辣根过氧化物酶（HRP）的新型H2 O2生物传感器。DNA通过其5′端连接到ZrO 2上，这为各种生物分子的固定提供了微环境，并促进了HRP与电极表面之间的电子转移。在优化的条件下，该传感器对H2 O2的检测线性范围为3.5μM ~ 10 mM，检测限为0.8μM，信噪比为3。此外，所开发的生物传感器显示出可接受的稳定性和重复性。重要的是，可以进一步开发用于固定其他蛋白质和生物化合物的分析方法。
A novel H2O2biosensor is described which is based on immobilization of horseradish peroxidase (HRP) on DNA/electrodeposited, ZrO2/modified, gold electrode. The DNA is attached via its 5′ end to ZrO2and this provides a microenvironment for the immobilization of various biomolecules and promotes electron transfer between HRP and the electrode surface. Under optimized conditions, the biosensor reduced H2O2linearly between 3.5μM and 10 mM with a detection limit of 0.8μM at a signal-to-noise ratio of 3. In addition, the developed biosensor shows an acceptable stability and repeatability. Importantly, the analytical methodology could be further developed for the immobilization of other proteins and biocompounds.