Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology
Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology
复制标题
合成生物学广谱启动子的构建和表征
DOI：
10.1021/acssynbio.7b00258
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Kang, Zhen
中科院分区：
文献类型：
--
作者：
Yang, Sen;Liu, Qingtao;Kang, Zhen
Characterization of genetic circuits and biosynthetic pathways in different hosts always requires promoter substitution and redesigning. Here, a strong, broad-spectrum promoter, P-bs, for Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae was constructed, and it was incorporated into the minimal E. coli-B. subtilis-S. cerevisiae shuttle plasmid pEBS (5.8 kb). By applying a random mutation strategy, three broad-spectrum promoters P-bs1, P-bs2, and P-bs3, with different strengths were generated and characterized. These broad-spectrum promoters will expand the synthetic biology toolbox for E. coli, B. subtilis, and S. cerevisiae.
