LYSOSOMAL RESPONSE OF A MURINE MACROPHAGE-LIKE CELL-LINE PERSISTENTLY INFECTED WITH COXIELLA-BURNETII
LYSOSOMAL RESPONSE OF A MURINE MACROPHAGE-LIKE CELL-LINE PERSISTENTLY INFECTED WITH COXIELLA-BURNETII
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DOI：
10.1128/iai.40.3.1155-1162.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
BACA, OG
中科院分区：
文献类型：
--
作者：
AKPORIAYE, ET;ROWATT, JD;BACA, OG
本文观察了小鼠巨噬细胞样瘤细胞株(J774)持续感染伯氏梭菌后的溶酶体反应。用酸性磷酸酶作为溶酶体标记物，发现持续感染伯氏弧菌的J774细胞发生了吞噬小体-溶酶体融合。这一观察结果得到了二氧化钍的验证，二氧化钍是一种电子密度高的化合物，隔离在次级溶酶体中。吞噬酶体中含有可复制的利氏杆菌。荧光分光光度分析表明，持续感染细胞的吞噬酶体液pH为酸性。为了将立克次体存活率与溶酶体功能联系起来，我们检测了感染细胞和未感染细胞中几种溶酶体酶的活性。在感染过程中，酸性磷酸酶和β-乙酰氨基葡萄糖苷酶的活性没有明显变化。感染细胞表现出略高的胞内溶菌酶、β-葡萄糖苷酸酶和β-半乳糖苷酶活性。
The lysosomal response of a murine macrophage-like tumor cell line (J774) during persistent infection with C. burnetii was examined. By using acid phosphatase as a lysosomal marker, phagosome-lysosome fusion was shown to occur in J774 cells persistently infected with C. burnetii. This observation was verified using thorium dioxide, an electron-dense compound that is sequestered in secondary lysosomes. The phagolysosomes contained viable replicating riskettsiae. Spectrofluorometric analysis indicated that the phagolysosomal pH of persistently infected cells was acidic. To correlate rickettsial survival with lysosome function, the activities of several lysosomal enzymes were assayed in infected and uninfected cells. Activities of acid phosphatase and .beta.-acetylglucosaminidase were not significantly altered during infection. Infected cells appeared to display slightly higher intracellular lysozyme, .BETA.-glucuronidase and .beta.-galactosidase activities.