Using cross-links to study ribosomal dynamics
Using cross-links to study ribosomal dynamics
复制标题
DOI：
10.1080/07391102.2004.10506962
复制
发表时间：
2004-04-01
影响因子：
4.4
通讯作者：
Mears, JA
中科院分区：
文献类型：
--
作者：
Ivanov, VI;Mears, JA
在静态核糖体及其大小亚基的 3D 模型发表后，接下来的任务之一是识别负责蛋白质合成过程中机械位移的可移动核糖体元件。对可用交联数据的统计分析使我们能够揭示核糖体中三组完全分离的运动：I，平均幅度为 10 埃； II，最丰富，中心在 20 埃，分布广泛；III，稀疏，距离大，可达 95 埃。最后一组III包括元素，如L7/12-茎和L1-突起，它们在晶体学或电子显微结构中采用不同的位置，以及指示流动性的相邻发夹88和89。我们证明交联方法可用于研究核糖体动力学，包括大规模功能运动，特别是估计哪些结构参与分子开关。
After publications of 3-D models of a static ribosome and its large and small subunits, one of the next tasks is to recognize movable ribosomal elements responsible for mechanical shifts during protein synthesis. Statistic analysis of available cross-linking data allowed us to reveal three well separated groups of motions in the ribosome: I, mean magnitude of 10Angstrom; II, most abundant, centered at 20Angstrom and of wide dispersion, and III, sparsely populated, with large distances up to 95Angstrom. The last group, III, comprises elements, like the L7/12-stalk and the L1-protuberance, that adopt different positions in crystallographic or electron micrographic structures, and neighboring hairpins 88 and 89, indicating mobility. We demonstrate that the cross-linking method can be applied to study ribosomal dynamics, including large-scale functional movements and, in particular, to estimate which structures participate in molecular switches.