Amperometric sensor for ethanol based on one-step electropolymerization of thionine-carbon nanofiber nanocomposite containing alcohol oxidase.
Amperometric sensor for ethanol based on one-step electropolymerization of thionine-carbon nanofiber nanocomposite containing alcohol oxidase.
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DOI：
10.1016/j.talanta.2007.09.023
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发表时间：
2007-12
期刊：
影响因子：
6.1
通讯作者：
Lina Wu;Mike McIntosh;Xueji Zhang;H. Ju
中科院分区：
文献类型：
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作者：
Lina Wu;Mike McIntosh;Xueji Zhang;H. Ju
硫氨酸与碳纳米纤维（CNF）具有较强的相互作用，利用其对碳纳米纤维进行非共价功能化，制备了分散性好的稳定的硫氨酸- CNF纳米复合材料。通过将硫氨酸-CNF纳米复合材料与乙醇氧化酶（AOD）进行简单的一步电化学聚合，在电极表面形成稳定的聚硫氨酸-CNF /AOD生物复合膜。基于生物复合膜对溶解氧还原的优异催化活性，提出了一种灵敏的乙醇生物传感器。乙醇生物传感器可监测2.0 ~ 252μM范围内的乙醇，检测限为1.7μM。该方法具有响应速度快、线性响应范围广、重现性和稳定性好等特点。结合CNF的催化活性和生物复合材料的前景与一步非人工技术相结合，有利于乙醇的灵敏测定，提高分析能力。
Thionine had strong interaction with carbon nanofiber (CNF) and was used in the non-covalent functionalization of carbon nanofiber for the preparation of stable thionine–CNF nanocomposite with good dispersion. With a simple one-step electrochemical polymerization of thionine–CNF nanocomposite and alcohol oxidase (AOD), a stable poly(thionine)–CNF/AOD biocomposite film was formed on electrode surface. Based on the excellent catalytic activity of the biocomposite film toward reduction of dissolved oxygen, a sensitive ethanol biosensor was proposed. The ethanol biosensor could monitor ethanol ranging from 2.0 to 252μM with a detection limit of 1.7μM. It displayed a rapid response, an expanded linear response range as well as excellent reproducibility and stability. The combination of catalytic activity of CNF and the promising feature of the biocomposite with one-step non-manual technique favored the sensitive determination of ethanol with improved analytical capabilities.