Identification of megakaryocytes, macrophages, and eosinophils in colonies of human bone marrow containing neurtophilic granulocytes and erythroblasts.
Identification of megakaryocytes, macrophages, and eosinophils in colonies of human bone marrow containing neurtophilic granulocytes and erythroblasts.
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DOI：
10.1182/blood.v53.5.1023.1023
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发表时间：
1979
期刊：
影响因子：
20.3
通讯作者：
A. Fauser;H. Messner
中科院分区：
文献类型：
--
作者：
A. Fauser;H. Messner
人骨髓中的多能造血祖细胞可以通过其在培养物中产生含有一种以上造血分化谱系的集落的能力来鉴定。在植物血凝素 (PHA-LCM) 和促红细胞生成素存在下，白细胞条件培养基支持这些混合集落的生长。由于它们由无色细胞和具有血红蛋白典型的红色外观的细胞组成，因此可以通过直接显微镜观察轻松识别它们。通过细胞离心制备来自6个个体的73个个体混合集落用于进一步的细胞学检查。通过酸性磷酸酶阳性反应鉴定，除了中性粒细胞和成红细胞外，73 个集落中的 16 个集落中还存在巨核细胞。在 73 个集落中，有 24 个集落发现了巨噬细胞，6 个集落中发现了嗜酸性粒细胞。
Pluripotent hemopoietic progenitors in human bone marrow can be identified by their ability to give rise in culture to colonies that contain more than one lineage of hemopoietic differentiation. Growth of these mixed colonies is supported by media conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) and erythropoietin. They can be readily recognized by direct microscopic observation because of their composition of colorless cells and cells with a red appearance typical for hemoglobin. Seventy-three individual mixed colonies from 6 individuals were prepared by cytocentrifugation for further cytological examination. In addition to neutrophilic granulocytes and erythroblasts, megakaryocytes were present in 16 of the 73 colonies, as identified by positive reaction for acid phosphatase. Macrophages were found in 24 and eosinophils in 6 of the 73 colonies.