Osteogenic differentiation of mesenchymal stem cells in self-assembled peptide-amphiphile nanofibers
Osteogenic differentiation of mesenchymal stem cells in self-assembled peptide-amphiphile nanofibers
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DOI：
10.1016/j.biomaterials.2006.03.030
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发表时间：
2006-08-01
期刊：
影响因子：
14
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Hosseinkhani, Hossein;Hosseinkhani, Mohsen;Tabata, Yasuhiko
研究了间充质干细胞（MSC）在由肽-两亲物（PA）分子自组装形成的纳米纤维三维（3-D）网络中的增殖和分化。PA通过标准固相化学合成，其以肽的NH 2末端的烷基化结束。肽段设计中还包括精氨酸-甘氨酸-天冬氨酸（RGD）序列。通过将细胞悬浮液与PA的稀水溶液混合，形成三维纳米纤维网络。扫描电子显微镜（SEM）观察显示，形成的纤维组装体具有极高的纵横比和高表面积。当将大鼠MSC接种到具有或不具有RGD的PA纳米纤维中时，在包括RGD的PA纳米纤维中观察到更多数量的细胞附着。当测量以评估MSC的成骨分化时，碱性磷酸酶（ALP）活性和骨钙素含量对于包含RGD的PA纳米纤维与不含RGD的PA纳米纤维相比变得最大，尽管这两个值与静态组织培养板（2-D培养）中的值相比显著更高。我们的结论是，PA纳米纤维作为细胞支架影响MSC的附着，增殖和成骨分化。(c)2006爱思唯尔有限公司保留所有权利。
The proliferation and differentiation of mesenchymal stem cells (MSC) was investigated in a three dimensional (3-D) network of nanofibers formed by self-assembly of peptide-amphiphile (PA) molecules. PA was synthesized by standard solid phase chemistry that ends with the alkylation of the NH2 terminus of the peptide. The sequence of arginine-glycine-aspartic acid (RGD) was included in peptide design as well. A 3-D network of nonofibers was formed by mixing cell suspensions in media with dilute aqueous solution of PA. Scanning electron microscopy (SEM) observation revealed the formation of fibrous assemblies with an extremely high aspect ratio and high surface areas. When rat MSC were seeded into the PA nanofibers with or without RGD, larger number of cells attached was observed in the PA nanofibers including RGD. When measured to evaluate the osteogenic differentiation of MSC, the alkaline phosphatase (ALP) activity and osteocalcin content became maximum for the PA nanofibers including RGD compared with those without RGD, although both the values were significantly higher compared with those in the static tissue culture plate (2-D culture). We concluded that the attachment, proliferation, and osteogenic differentiation of MSC were influenced by PA nanofibers as the cell scaffold. (c) 2006 Elsevier Ltd. All rights reserved.