Lactobacillus rhamnosus Strain LRH05 Intervention Ameliorated Body Weight Gain and Adipose Inflammation via Modulating the Gut Microbiota in High-Fat Diet-Induced Obese Mice
Lactobacillus rhamnosus Strain LRH05 Intervention Ameliorated Body Weight Gain and Adipose Inflammation via Modulating the Gut Microbiota in High-Fat Diet-Induced Obese Mice
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DOI：
10.1002/mnfr.202100348
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发表时间：
2021-12-02
影响因子：
5.2
通讯作者：
Lin, Jin-Seng
中科院分区：
文献类型：
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作者：
Chen, Yung-Tsung;Chiou, Shiou-Yun;Lin, Jin-Seng
研究范围本研究旨在探讨特定益生菌菌株抑制高脂饮食（HFD）喂养小鼠白色脂肪组织（WAT）脂肪生成和炎症反应的潜在机制。方法与结果对8株具有潜在益生菌候选的菌株进行了体外筛选。在体外筛选出具有较低甘油三酯表达的鼠李糖乳杆菌LRH 05（LRH 05）和罗伊氏乳杆菌LR 47（LR 47）。给小鼠分别喂食对照饮食（CD）、HFD或补充有10（9）CFU/小鼠/天剂量的LRH 05或LR 47的HFD，持续10周（n = 8）。结果表明，LRH 05而不是LR 47显著降低体重增加和WAT重量，以及改善肝脂肪变性和葡萄糖耐受不良。LRH 05调节Mogat 1、Igf-1、Mcp-1和F4/80 mRNA表达并减少WAT中的巨噬细胞浸润。LRH 05显示丁酸和丙酸产生细菌的增加，包括毛梭菌属、Romboutsia和梭杆菌属，这与粪便丙酸和丁酸水平的增加一致。结论LRH 05通过减少小鼠炎症巨噬细胞浸润和炎症相关基因表达，具有株特异性的抗炎作用。因此，LRH 05可以被认为是预防肥胖的潜在益生菌菌株。
Scope This study aims to investigate the underlying mechanism of a specific probiotic strain on suppression of adipogenesis and inflammatory response in white adipose tissue (WAT) of high-fat diet (HFD)-fed mice. Methods and Results Eight strains are screened in vitro for candidates of potential probiotics. Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 10(9) CFU per mouse per day for 10 weeks (n = 8), respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf-1, Mcp-1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid-producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels. Conclusion LRH05 shows a strain-specific effect on ameliorating the pro-inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation-related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity.