Immunocytochemical staining of cells in pleural and peritoneal effusions with a panel of monoclonal antibodies.
Immunocytochemical staining of cells in pleural and peritoneal effusions with a panel of monoclonal antibodies.
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使用一组单克隆抗体对胸腔和腹腔积液中的细胞进行免疫细胞化学染色。
DOI：
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发表时间：
1983
影响因子：
3.4
通讯作者：
D. Mason
中科院分区：
文献类型：
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作者：
A. Ghosh;A. Spriggs;J. Taylor‐Papadimitriou;D. Mason
使用免疫碱性磷酸酶(IAP)程序，对70例胸膜和腹腔液中的细胞沉淀物涂片进行了7种单抗的检测。这些病例被选为典型的细胞学类型，既有良性的也有恶性的，这样就可以评估该方法的诊断价值。所用抗体为2D1(抗白细胞)、Ca1、HMFG-2(抗乳脂球膜)、LE61和M73(均为抗中间丝抗体)、抗CEA和K92(抗角蛋白)。抗白细胞抗体可用于淋巴瘤和癌的鉴别。抗CEA抗体在80%的癌组织中呈阳性反应，与其他类型的细胞均无反应。在95%的癌组织中，CA1与部分细胞呈阳性反应，但有2例间皮细胞呈阳性反应。抗乳脂肪球膜抗体在癌组织中呈强阳性反应，但在30%的良性胸腔积液中也可见于间皮细胞。抗角蛋白抗体仅在一小部分病例中与恶性细胞发生反应。抗上皮中间丝抗体与良性间皮细胞和癌细胞反应同样强烈，而与淋巴瘤细胞呈阴性反应。结论：选择一组合适的单抗对鉴别浆膜腔积液中的肿瘤细胞具有重要价值。
A panel of seven monoclonal antibodies was applied to smears of cell deposit from 70 pleural and peritoneal fluids, using an immunoalkaline phosphatase (IAP) procedure. The cases were chosen to show typical cytological patterns, both benign and malignant, and in this way the diagnostic value of the method could be assessed. The antibodies used were 2D1 (anti-leucocyte), Ca 1, HMFG-2 (anti-milk fat globule membrane), LE61 and M73 (both anti-intermediate filament antibodies), anti-CEA, and K92 (anti-keratin). The anti-leucocyte antibody was found useful for distinguishing lymphoma from carcinoma. Anti-CEA gave positive reactions in 80% of carcinoma cases and was not seen to react with any other cell types. Ca 1 was positive with some cells in 95% of carcinoma cases, but mesothelial cells reacted with it in two cases. A strong reaction with the anti-milk fat globule membrane antibody was very constant in carcinoma but was also seen in mesothelial cells in 30% of benign effusions. The anti-keratin reacted with malignant cells in only a small proportion of cases. The antibodies against epithelial intermediate filaments reacted equally strongly with benign mesothelial cells and carcinoma cells, but gave negative reactions with lymphoma cells. It is concluded that a suitably chosen panel of monoclonal antibodies can be of great value in identifying neoplastic cells in serous effusions.
CEA、ZGM 和 EMA 在胸腔和腹腔积液细胞中的定位：初步研究。
DOI：
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发表时间：
1980
期刊：
Investigative & cell pathology
影响因子：
--
作者：
O'Brien,MJ;Kirkham,SE;Burke,B;Ormerod,M;Saravis,CA;Gottlieb,LS;Neville,AM;Zamcheck,N
通讯作者：
Zamcheck,N