Evaluation of a microbial muramidase supplementation on growth performance, apparent ileal digestibility, and intestinal histology of broiler chickens
Evaluation of a microbial muramidase supplementation on growth performance, apparent ileal digestibility, and intestinal histology of broiler chickens
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DOI：
10.3382/ps/pey556
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发表时间：
2019-05-01
期刊：
影响因子：
4.4
通讯作者：
Zentek, J.
中科院分区：
文献类型：
--
作者：
Boroojeni, F. Goodarzi;Maenner, K.;Zentek, J.
本研究通过测定营养物质的表观回肠消化率（AID）、研究肠道组织形态学和量化生长性能，评价了不同包合水平的微生物胞壁酶（胞壁酶007，DSM Nutritional Products）对胃肠道功能的影响。在玉米-小麦-大豆试验日粮中添加不同剂量的溶菌酶（L，25，000 LSU（F）/kg）、中剂量（M，35，000 LSU（F）/kg）和高剂量（H，45，000 LSU（F）/kg），饲喂肉仔鸡35 d。试验结束后，测定乙醚提取物（EE）、粗蛋白（CP）、钙（Ca）、磷（P）的AID，并取空肠中段和回肠进行组织形态学观察。使用GLM程序对数据进行ANOVA分析。正交多项式对比用于评估不同水平的溶菌酶的线性和二次效应。在试验结束时，补充胞壁酶007线性增加体重增加，降低饲料转化率（FCR）（P 0.05）。在肉鸡日粮中添加溶菌酶也线性增加了欧洲家禽的效率因子（P 0.05）。在肉鸡日粮中添加溶菌酶线性增加了CP、EE和P的AID（P 0.05），H组EE和CP的AID高于C组（P 0.05）。补充微生物溶菌酶线性增加回肠绒毛长度与隐窝深度的比值，并减少回肠CD 45细胞的数量（P 0.05）。与C组相比，M组和H组回肠中CD 45细胞数量减少（P0.05）。综上所述，本研究的结果表明，在肉鸡日粮中加入微生物溶菌酶可以提高肉鸡关键营养素的AID，改善肉鸡的生长性能。因此，在肉鸡日粮中添加微生物溶菌酶可以被认为是改善胃肠道功能的一个有趣的前景。导致这些改善的生物学机制需要进一步研究。
The current study evaluated the effects of different inclusion levels of microbial muramidase (Muramidase 007, DSM Nutritional Products) on gastrointestinal functionality by determination of apparent ileal digestibility (AID) of nutrients, investigation of intestinal histomorphology, and quantification of resulting growth performance. Four maize-wheat-soybean experimental diets were produced without (C) and with different dosages of muramidase: low (L, 25,000 LSU(F)/kg), medium (M, 35,000 LSU(F)/kg), and high (H, 45,000 LSU(F)/kg); diets were fed to broilers for 35 d. At the end of the experiment, AID of ether extract (EE), crude protein (CP), Ca, and P were determined and samples of the mid-jejunum and -ileum were collected for histomorphological observations. Data were subjected to ANOVA analysis using the GLM procedure. Orthogonal polynomial contrasts were used to assess linear and quadratic effects of different levels of the muramidase. At the end of the trial, Muramidase 007 supplementation linearly increased body weight gain and decreased feed conversion ratio (FCR) (P 0.05). Adding the muramidase to broiler diets also linearly increased the European poultry efficiency factor (P 0.05). Inclusion of the muramidase in broiler diets linearly increased AID of CP, EE, and P (P 0.05), and the H group had a higher AID of EE and CP compared to C group (P 0.05). Microbial muramidase supplementation linearly increased ileal villus length to crypt depth ratio and decreased the number of ileal CD45 cells (P 0.05). Broilers fed M and H diets had fewer number of CD45 cells in the ileum compared to those in C group (P 0.05). In conclusion, the results of the present study demonstrated that inclusion of the microbial muramidase in broiler diets could increase AID of key nutrients and improve growth performance in broilers. Adding microbial muramidase to broiler diets can therefore be considered as an interesting prospect to improve gastrointestinal functionality. Biological mechanisms causing these improvements need to be studied further.