ADENOVIRUS 5 DNA SEQUENCES PRESENT AND RNA SEQUENCES TRANSCRIBED IN TRANSFORMED HUMAN-EMBRYO KIDNEY-CELLS (HEK-AD-5 OR 293)
ADENOVIRUS 5 DNA SEQUENCES PRESENT AND RNA SEQUENCES TRANSCRIBED IN TRANSFORMED HUMAN-EMBRYO KIDNEY-CELLS (HEK-AD-5 OR 293)
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DOI：
10.1016/0042-6822(79)90476-8
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发表时间：
1979-01-01
期刊：
影响因子：
3.7
通讯作者：
WEINMANN, R
中科院分区：
文献类型：
--
作者：
AIELLO, L;GUILFOYLE, R;WEINMANN, R
分析了从剪切的腺病毒 5 片段转化的人胚胎肾细胞系 (293) 中存在的病毒 DNA 序列。通过转化细胞 DNA 和用 HindIII 获得的标记腺病毒 5 限制酶片段的复性动力学，我们确定病毒基因组左端 12% 有 4-5 个拷贝，细胞和病毒基因组右端 9% 大约有 1 个拷贝/细胞。对该细胞系分离的细胞核中转录的RNA序列的分析表明，仅检测到来自左端序列的转录物。这些病毒序列的表达是由 DNA 依赖性 RNA 聚合酶 II 介导的。使用限制酶对 293 个细胞的病毒 DNA 进行分析表明，病毒 DNA 已整合并位于细胞基因组的 2 个位点上。这种简单的排列在多次细胞传代后仍保持稳定。
The viral DNA sequences present in the human embryo kidney cell line (293) transformed from sheared adenovirus 5 fragments were analyzed. By renaturation kinetics of transformed cell DNA and labeled adenovirus 5 restriction enzyme fragments obtained with HindIII we determined that there are 4-5 copies, cell of the left 12% of the viral genome and approximately 1 copy/cell of 9% of the right end of the viral genome. Analysis of the RNA sequences transcribed in nuclei isolated from this cell line indicated that only transcripts from the sequences of the left end were detected. The expression of these viral sequences was mediated by DNA-dependent RNA polymerase II. Analysis of the viral DNA from 293 cells using restriction enzymes reveals that the viral DNA is integrated and located at 2 sites on the cellular genome. This simple arrangement is stable through several cell passages.