Bovine lentivirus induces early transient B-cell proliferation in experimentally inoculated cattle and appears to be pantropic
Bovine lentivirus induces early transient B-cell proliferation in experimentally inoculated cattle and appears to be pantropic
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牛慢病毒在实验接种的牛中诱导早期短暂的 B 细胞增殖，并且似乎具有泛嗜性
作者：
C. Whetstone;D. Suarez;J. Miller;B. Pesch;J. Harp
牛免疫缺陷样病毒（BIV）于1972年首次分离（M. J. VanDerMaaten等人，J. Natl. 49：1649-1657，1972）。在使用原始BIV R29分离株的研究中，已经对BIV的分子特征进行了大量研究;然而，认为R29是减毒的，因为它不再引起实验感染牛的单核细胞数量增加或淋巴结可检测到的增大。BIV感染后宿主细胞嗜性和宿主外周血淋巴细胞群的变化尚不清楚。最近，我们分离并鉴定了BIV的野外分离物FL 112（D. L.苏亚雷斯等人，J. Virol. 67：5051-5055，1993），其在实验感染的牛中引起瞬时单核细胞淋巴细胞增多。在本研究中，牛接种BIV FL 112，流式细胞术数据显示，BIV引起B细胞淋巴细胞增多，其他单核细胞群（包括CD 3+、CD 4+和CD 8+细胞）无一致的显著变化。细胞分选和PCR扩增结果表明，BIV可能是泛嗜性的。接种后3 - 6天以及接种后2.5年，CD 3+、CD 4+、CD 8+和B细胞、单核细胞和WC 1细胞（γ/δ T细胞、空细胞）中存在前病毒DNA。
Bovine immunodeficiency-like virus (BIV) was first isolated in 1972 (M. J. VanDerMaaten et al., J. Natl. Cancer Inst. 49:1649-1657, 1972). Much work has been done on the molecular characterization of BIV in studies using the original BIV R29 isolate; however, R29 is believed to be attenuated since it no longer causes either mononuclear cell number increases or detectable enlargement of lymphatic nodules in experimentally infected cattle. The host cell tropism and changes in host peripheral blood lymphocyte populations following infection with BIV are unknown. Recently, we isolated and characterized a field isolate of BIV, FL112 (D. L. Suarez et al., J. Virol. 67:5051-5055, 1993) that causes a transient, mononuclear cell lymphocytosis in experimentally infected cattle. In the present study, cattle were inoculated with BIV FL112, and data from flow cytometry showed that BIV causes a B-cell lymphocytosis with no consistent, significant changes in other mononuclear cell populations, including CD3+, CD4+, and CD8+ cells. Cell sorting and PCR amplification were used to show that BIV may be pantropic. Proviral DNA was present in CD3+, CD4+, CD8+, and B-cells, monocytes, and WC1 cells (gamma/delta T cells, null cells) by 3 to 6 days postinoculation and also at 2.5 years postinoculation.
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通过在实验感染的小牛中进行巢式聚合酶链反应测试，改进了牛慢病毒的早期和长期检测。
DOI：
--
发表时间：
1995
期刊：
American journal of veterinary research.
影响因子：
--
作者：
Suarez,DL;VanderMaaten,MJ;Whetstone,CA
通讯作者：
Whetstone,CA
实验性感染牛免疫缺陷样病毒的牛的单核细胞功能。
影响因子：
1.8
作者：
Rovid,AH;Carpenter,S;Roth,JA
通讯作者：
Roth,JA
影响因子：
1.8
作者：
DUA, N;REUBEL, G;PEDERSEN, NC
通讯作者：
PEDERSEN, NC
牛类免疫缺陷病毒感染对实验感染牛免疫功能的影响。
影响因子：
1.8
作者：
Flaming,K;vanderMaaten,M;Whetstone,C;Carpenter,S;Frank,D;Roth,J
通讯作者：
Roth,J