The genetic program of hematopoietic stem cells
The genetic program of hematopoietic stem cells
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DOI：
10.1126/science.288.5471.1635
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发表时间：
2000-06-02
期刊：
影响因子：
56.9
通讯作者：
Lemischka, IR
中科院分区：
文献类型：
--
作者：
Phillips, RL;Ernst, RE;Lemischka, IR
血细胞的产生来源于一种罕见的多能、自我更新的干细胞。进行了全基因组基因表达分析，以确定干细胞中的调控途径及其全局遗传程序。应用生物信息学和阵列杂交技术对高纯度的小鼠胎肝干细胞亚群互补DNA文库进行了分析。已经鉴定的几千种基因产物中有很大一部分对应于以前未描述的具有暗示调节功能的性质的分子。在面向生物过程的数据库中可获得的完整数据代表造血干细胞的分子表型。
Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA Libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.