Mir-142-5p as an indicator of autoimmune processes in childhood idiopathic nephrotic syndrome and as a part of MicroRNAs expression panels for its diagnosis and prediction of response to steroid treatment
Mir-142-5p as an indicator of autoimmune processes in childhood idiopathic nephrotic syndrome and as a part of MicroRNAs expression panels for its diagnosis and prediction of response to steroid treatment
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DOI：
10.1016/j.molimm.2021.11.004
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发表时间：
2021-11-16
影响因子：
3.6
通讯作者：
Gayed, Eman Masoud Abd El
中科院分区：
文献类型：
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作者：
Bayomy, Noha Rabie;Alfottoh, Wafaa Moustafa Abo;Gayed, Eman Masoud Abd El
背景：肾病综合征（NS）是儿童中最常见的肾小球疾病。肾活检是诊断和跟踪儿童 NS 的最精确的方法；然而，这是一种侵入性手术，具有潜在的并发症。因此，该疾病迫切需要早期无创诊断和预后指标以及新的治疗靶点。目的：评估外周血中 miR-142-5p 表达作为 NS 儿童自身免疫过程的指标，以及差异 microRNA (miR) 表达和表达组在诊断和预测 NS 儿童对类固醇治疗反应中的作用。方法：八十 (80) 名 NS 儿童和 100 名年龄和性别相匹配的受试者作为对照，构成本病例对照研究的研究样本。通过实时 PCR 测量所有入组儿童的 miR-142-5p、miR-191、miR-181-5p、miR-30a-5p 和 miR-50a-5p 表达。我们评估了不同 MicroRNA 组合的敏感性和准确性。结果：NS患儿中miR-142-5p、miR-191、miR-181-5p、miR-30a-5p和miR-150a-5p表达量显着高于对照组。激素抵抗型和激素敏感型 NS 儿童的 5 种 mRNA 差异表达存在显着差异。在选定的 5 个 microRNA 中，miR-142a-5p 是最好的，可以很好地区分 NS 儿童并预测类固醇抵抗（AUC 分别为 0.965 和 1.00），表明自身免疫过程在 NS 发病机制和类固醇抵抗中可能发挥作用。 （miR-142a-5p、miR-181a-5p 和 miR-30a-5p）是诊断新 NS 病例和预测类固醇耐药的最佳表达组合。结论：microRNA 表达，无论是差异表达还是整体表达，对于儿童 NS 的早期诊断都很重要，并且可能为这些患者对类固醇治疗的反应提供非侵入性线索。 （miR142a-5p、miR-181-5p 和 miR-30a-5p）小组是涵盖新病例诊断和类固醇治疗反应预测的最佳小组。自身免疫在 NS 发病机制和对类固醇治疗的抵抗中发挥重要作用。
Background: Nephrotic syndrome (NS) is the most frequent glomerular disease among children. Renal biopsy is the most precise procedure for diagnosing and following childhood NS; however, it is an invasive procedure with potential complications. As a result, early non-invasive diagnostic and prognostic indicators and new treatment targets are urgently needed for this disease. Purpose: To assess the miR-142-5p expression in peripheral blood as an indicator of the autoimmune processes in children with NS and the role of differential microRNAs (miR) expression and expression panels in diagnosing and predicting the response to steroid treatment in children with NS. Methods: Eighty (80) children with NS and 100 subjects matched for age and gender used as controls constitute the study sample in this case-control study. MiR-142-5p, miR-191, miR-181-5p, miR-30a-5p and miR-50a-5p expression are measured in all enrolled children by real-time PCR. We assessed the sensitivity and accuracy of different MicroRNAs panels. Results: miR-142-5p, miR-191, miR-181-5p, miR-30a-5p and miR-150a-5p expressions were significantly increased in the children with NS than controls. There was a significant difference in the five mRNAs differential expressions between steroid-resistant and steroid-sensitive children with NS. Of the selected five microRNAs, miR-142a-5p was the best to allow very good discrimination of the children with NS and predict steroid resistance (AUC = 0.965 and 1.00, respectively), suggesting the possible autoimmunity processes' role in the pathogenesis of NS and the resistance to steroids. The (miR-142a-5p with miR-181a-5p and miR-30a-5p) was the best expression panel to diagnose new NS cases and predict steroid resistance. Conclusions: microRNAs expressions, either differential or as a panel, are important for early diagnosing childhood NS and may provide a non-invasive clue for the response to steroid treatment in these patients. The (miR142a-5p, miR-181-5p, and miR-30a-5p) panel was the best one to cover both the diagnosis of the new cases and prediction of response to steroid treatment. Autoimmunity has an important role in NS pathogenesis and resistance to steroid treatment.