Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae).
Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae).
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DOI：
10.4238/2011.december.12.3
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
S. Yang;L. Wang;Y. Zhang;X. Liu;H. Lin;Z. Meng
中科院分区：
文献类型：
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作者：
S. Yang;L. Wang;Y. Zhang;X. Liu;H. Lin;Z. Meng
披针石斑鱼（Epinealphus lanceolatus）是我国台湾及沿海地区广泛养殖的重要经济鱼类。从富含CA的石斑鱼基因组文库中分离并鉴定了32个多态性微卫星位点。每个位点的等位基因数为3 ~ 7个，平均4.69个。每个位点的观测杂合度和期望杂合度分别为0.387 ~ 1.000和0.377 ~ 0.843。6个位点显著偏离Hardy-Weinberg平衡。经序贯Bonferroni校正后，只有两个位点偏离了Hardy-Weinberg平衡，没有发现任何一对位点之间存在连锁不平衡。这些微卫星标记可为石斑鱼群体遗传学研究提供有用的工具。
An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed and expected heterozygosities per locus varied from 0.387 to 1.000 and from 0.377 to 0.843, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium. After sequential Bonferroni's correction, only two loci showed deviation from Hardy-Weinberg equilibrium, and no linkage disequilibrium was found between any pair of loci. These microsatellites can be useful tools for the study of population genetics in the giant grouper.