Hydrolysis of phytic acid by intrinsic plant and supplemented microbial phytase (Aspergillus niger) in the stomach and small intestine of minipigs fitted with re-entrant cannulas 3.: Hydrolysis of phytic acid (IP6) and occurrence of hydrolysis products (IP5, IP4, IP3 and IP2)
Hydrolysis of phytic acid by intrinsic plant and supplemented microbial phytase (Aspergillus niger) in the stomach and small intestine of minipigs fitted with re-entrant cannulas 3.: Hydrolysis of phytic acid (IP6) and occurrence of hydrolysis products (IP5, IP4, IP3 and IP2)
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DOI：
10.1046/j.1439-0396.2001.00337.x
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发表时间：
2001-12-01
影响因子：
2.7
通讯作者：
Drochner, W
中科院分区：
文献类型：
--
作者：
Rapp, C;Lantzsch, HJ;Drochner, W
本研究采用6头体重40-50 kg的小型猪（初始体重40-50 kg），分别在幽门后30 cm的十二指肠（动物1、4、5和6）和回盲部（动物1、2和3）前5 cm的回盲部（动物1、2和3）处安装再入式瘘管，研究了内在植物（小麦）和补充微生物植酸酶（黑曲霉）对胃和小肠中植酸水解的影响。饲粮处理如下：(1)饲粮1为玉米基饲粮（43 U植酸酶/kg干物质）；(2)饲粮2，饲粮1添加微生物植酸酶（818 U/kg DM）；(3)饲粮3，以小麦为基础的饲粮（1192 U/kg DM）。在07 30h和19 30h，每只动物分别饲喂350 g饲粮和1050 ml去离子水。在饲喂后12 h内连续收集完整的Digcsta。饲粮1、2和3对动物1、4、5和6的胃近端IP6平均水解率分别为9.0、77.2和66.2%。微生物植酸酶水解植酸盐的效果明显优于小麦植酸酶。动物1、2和3回肠远端分别测定的饲粮中IP6在胃和小肠的平均水解率分别为19.0%、62.6和64.6%，均低于仅胃处理的水平。不同实验动物在胃中水解植酸酶的能力存在差异，这与饲料中植酸酶的存在和来源无关。饲喂饲粮2和饲粮3时，十二指肠和回肠食糜均产生了大量的水解产物；然而，只有在摄入饮食1后才检测到微量。与饲料处理无关，检测到4种IP5异构体，但含量不同。
Hydrolysis of phytate in the stomach and the small intestine as influenced by intrinsic plant (wheat) and supplemented microbial phytase (Aspergillus niger) were investigated with six minipigs (40-50 kg initial body weight) fitted with re-entrant cannulas in the duodenum, 30 cm posterior to the pylorus (animals 1, 4, 5 and 6) and ileocecal re-entrant cannulas, 5 cm prior the ileocecal junction (animals 1, 2 and 3), respectively. Dietary treatments were as follows: (1) diet 1, a corn-based diet [43 U phytase/kg dry matter (DM)]; (2) diet 2, diet 1 supplemented with microbial phytase (818 U/kg DM); and (3) diet 3, a wheat-based diet (1192 U/kg DM). At 07 30 h and 19 30 h, each animal was fed 350 g diet mixed with 1050 ml de-ionized water. Digcsta were collected continuously and completely during a 12-h period after feeding. Mean hydrolysis rates of IP6 in the stomach as measured at the proximal duodenum of animals 1, 4, 5 and 6 were 9.0, 77.2 and 66.2% for diet 1, 2 and 3, respectively. Microbial phytase was much more effective in phytate hydrolysis than wheat phytase. Mean IP6 hydrolysis rates of the respective diets in the stomach and small intestine as measured at the distal ileum of animals 1, 2 and 3 were 19.0, 62.6 and 64.6% and were lower than treatment means of the stomach only. Differences existed between experimental animals with respect to their ability to hydrolyse IP, in the stomach independent of the presence and source of dietary phytase. Considerable amounts of hydrolysis products occurred in both the duodenal and ileal digesta when diets 2 and 3 were fed; however, only traces were determined after ingestion of diet 1. Independent of dietary treatment, four IP5 isomers were detected, but in different amounts.