Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break.
Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break.
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遗传证据表明，酿酒酵母 HIS4 基因座的减数分裂重组热点并不代表对称处理的双链断裂的位点。
DOI：
10.1093/genetics/134.1.5
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Porter,SE;White,MA;Petes,TD
在酵母中，Rap1蛋白结合到HIS4基因的5‘端和BIK1基因的3’端之间的位点，刺激两侧位点的减数分裂重组。通过使用含有位于HIS4和BIK1突变的菌株，我们发现Rap1结合刺激的大多数重组事件涉及HIS4或BIK1，而不是包括两个位点的双向事件。异常分离的模式表明，大多数rap1刺激的重组事件并不代表位于rap1结合位点的双链DNA断裂的对称加工。
In the yeast Saccharomyces cerevisiae, the binding of the Rap1 protein to a site located between the 5' end of the HIS4 gene and the 3' end of BIK1 stimulates meiotic recombination at both flanking loci. By using strains that contain mutations located in HIS4 and BIK1, we found that most recombination events stimulated by the binding of Rap1 involve HIS4 or BIK1, rather than bidirectional events including both loci. The patterns of aberrant segregation indicate that most of the Rap1-stimulated recombination events do not represent the symmetric processing of a double-strand DNA break located at the Rap1-binding site.
来自 III 号染色体上 C1G 的 11,953 bp 片段的完整序列包含四个新的开放阅读框。
影响因子：
2.6
作者：
M. R. Rad;K. Lützenkirchen;G. Xu;U. Kleinhans;C. Hollenberg
通讯作者：
C. Hollenberg