A Synbiotic with Tumor Necrosis Factor-α Inhibitory Activity Ameliorates Experimental Jejunoileal Mucosal Injury.
A Synbiotic with Tumor Necrosis Factor-α Inhibitory Activity Ameliorates Experimental Jejunoileal Mucosal Injury.
复制标题
具有肿瘤坏死因子-α 抑制活性的合生元可改善实验性空肠粘膜损伤。
DOI：
10.1155/2018/9184093
复制
发表时间：
2018
影响因子：
--
通讯作者：
Ikuta,Tohru
中科院分区：
文献类型：
--
作者：
Takahashi,Ryoki;Noguchi,Takayasu;Mizoguchi,Yoko;Shimoyama,Tadashi;Nakazawa,Teruko;Ikuta,Tohru
尽管最近开发了用于炎症性肠病（IBD）的生物改性剂，但由于其可忽略的不良反应，发酵药物仍受到相当大的关注。我们以前表明，合生元肠道工作片（GWT）减轻实验性结肠炎。在这里，我们表明，GWT是能够改善空回肠粘膜损伤，这是常见的IBD。我们通过注射甲氨蝶呤（MTX）在大鼠中建立了实验性空回肠粘膜炎，甲氨蝶呤（MTX）可增加肠道通透性，这是IBD的标志性发现。对注射MTX的大鼠给予GWT可通过逆转粘膜中的绒毛缩短、隐窝丢失和杯状细胞消耗来恢复肠道完整性。GWT还降低了髓过氧化物酶和脂质过氧化物酶的活性，并增加了超氧化物歧化酶的活性，这对维持肠道功能至关重要。我们进一步发现，GWT抑制巨噬细胞中肿瘤坏死因子-α（TNF-α）和白细胞介素-12（IL-12）的mRNA表达，并降低实验性结肠炎标本中TNF-αmRNA表达，这与VSLI #3增强TNF-α产生相反。总之，目前和以前的动物研究清楚地证明了GWT在化学诱导的小肠结肠炎中的保护作用。克罗恩病是一种众所周知的IBD，可以影响肠道的任何部分，这些结果表明GWT可能用作IBD的新型治疗或维持疗法。
Despite the recent development of biological modifiers for inflammatory bowel diseases (IBD), there continues to be considerable interest in fermented medicines because of its negligible adverse effects. We previously showed that the synbiotic Gut Working Tablet (GWT) alleviates experimental colitis. Here we show that GWT is capable of ameliorating jejunoileal mucosal injury, which is frequently seen with IBD. We created experimental jejunoileal mucositis in rats by injection of methotrexate (MTX) which increases intestinal permeability, a hallmark finding of IBD. Administering GWT to MTX‐injected rats restored intestinal integrity by reversing villi shortening, crypt loss, and goblet cell depletion in the mucosa. Also GWT reduced activities of myeloperoxidase and lipid peroxidase and increased superoxide dismutase activity, which is critical for maintaining intestinal function. We further found that GWT suppressed mRNA expression of tumor necrosis factor‐α(TNF‐α) and interleukin‐12 (IL‐12) in macrophage and reduced TNF‐αmRNA expression in specimens with experimental colitis, which is in contrast to VSL#3 that enhanced TNF‐αproduction. Together, the current and previous animal studies clearly demonstrate the protective role of GWT in chemically induced enterocolitis. Crohn’s disease, a well‐known IBD, can affect any portion of the intestine, and these results suggest that GWT may be useful as a novel therapeutic or maintenance therapy for IBD.