Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation
Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation
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DOI：
10.2478/s11534-011-0018-3
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发表时间：
2011-02
影响因子：
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通讯作者：
S. Vertu;J. Fluegge;J. Delaunay;O. Haeberlé
中科院分区：
文献类型：
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作者：
S. Vertu;J. Fluegge;J. Delaunay;O. Haeberlé
层析衍射显微镜是一种技术，它允许在三维空间中成像透明的活体标本而无需染色。它通常在两种配置中实现，通过旋转样品照明保持样品固定，或通过使用固定照明旋转样品。在一阶玻恩近似下，可以用旋转照明方法映射的频域的体积具有“甜甜圈”的形状，其表现出所谓的“缺失锥”的非捕获频率，负责透射显微镜的强分辨率各向异性特性。当旋转样品时，分辨率几乎是各向同性的，但是捕获的频率集仍然显示出缺失的部分，其形状类似于苹果核。此外，它的最大扩展相比，减少了旋转照明的断层扫描。我们提出了各种配置的层析衍射显微镜，其中联合收割机结合这两种方法，并旨在获得高和各向同性的分辨率。我们用模拟说明了这些配置的预期成像性能。
Tomographic Diffractive Microscopy is a technique, which permits to image transparent living specimens in three dimensions without staining. It is commonly implemented in two configurations, by either rotating the sample illumination keeping the specimen fixed, or by rotating the sample using a fixed illumination. Under the first-order Born approximation, the volume of the frequency domain that can be mapped with the rotating illumination method has the shape of a “doughnut”, which exhibits a so-called “missing cone” of non-captured frequencies, responsible for the strong resolution anisotropy characteristic of transmission microscopes. When rotating the sample, the resolution is almost isotropic, but the set of captured frequencies still exhibits a missing part, the shape of which resembles that of an apple core. Furthermore, its maximal extension is reduced compared to tomography with rotating illumination. We propose various configurations for tomographic diffractive microscopy, which combine both approaches, and aim at obtaining a high and isotropic resolution. We illustrate with simulations the expected imaging performances of these configurations.