Viruses Infecting Cassava in Kenya.
Viruses Infecting Cassava in Kenya.
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感染肯尼亚木薯的病毒。
DOI：
10.1094/pdis.2004.88.1.17
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发表时间：
2004
期刊：
影响因子：
4.5
通讯作者：
E. Maiss
中科院分区：
文献类型：
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作者：
H. Wéré;S. Winter;E. Maiss
在肯尼亚主要的木薯种植区对木薯病毒进行了调查。采用生物学、电子显微镜、酶联免疫吸附试验和聚合酶链式反应等方法，对185个病株叶片样品和62个病株茎段进行了分析。所有来自肯尼亚西部的样本在单一感染或混合感染中都含有木薯花叶病毒(非洲木薯花叶病毒[ACMV]、东非木薯花叶病毒[EACMV]和乌干达变种[EACMV-UG])。然而，所有来自海岸地区的样本都只感染了EACMV，一种巨型病毒。此外，15个样本混合感染了EACMV和其他三种迄今未确定的丝状病毒。病毒长度分别为200、500、650和750 nm。除了在粗汁液中看到的杆状和一些弯曲的病毒外，还观察到了风车，这表明一些病毒可能与马铃薯Y病毒科有关。这些病毒在烟草上引起的症状非常严重，常常导致受试植株死亡约50%。回接木薯可获得100%的侵染。这一发现提供的证据表明，除了在非洲引起严重木薯病害的海绵病毒外，也存在丝状病毒，尽管它们的分布有限，但它们可能会在当地产生重大影响，而且最有可能与海绵病毒一样严重。讨论了这些发现的影响，并对未来的工作提出了建议。
A survey of cassava viruses was conducted in major cassava-growing regions of Kenya. A total of 185 leaf samples and 62 stem cuttings from plants with viral disease symptoms were collected and analyzed by biological, electron microscopy, enzyme-linked immunosorbent assay, and polymerase chain reaction. All samples from western Kenya had cassava begomoviruses (African cassava mosaic virus [ACMV], East African cassava mosaic virus [EACMV], and Uganda variant [EACMV-UG]) in either single or in mixed infection. However, all samples from the Coast region were infected with only EACMV, a begomovirus. In addition, 15 samples had mixed infections of EACMV and three other hitherto unidentified filamentous viruses. The viruses observed were 200, 500, 650, and 750 nm long, respectively. In addition to rod-shaped and some flexuous viruses, as seen in a crude sap preparation, pinwheels also were observed, indicating a possible association of some of the viruses with the Potyviridae family. The symptoms induced by these viruses in Nicotiana benthamiana were very severe and often caused about 50% death of the test plants. Back inoculation onto cassava resulted in 100% infections. This finding provides evidence that, other than begomoviruses that cause serious diseases of cassava in Africa, filamentous viruses also are present and, despite their limited distribution, they could reach local significance and, most probably, be as serious as begomoviruses. The implications of these findings are discussed and recommendations for future work suggested.