A surfactant-mediated sol-gel method for the preparation of molecularly imprinted polymers and its application in a biomimetic immunoassay for the detection of protein
A surfactant-mediated sol-gel method for the preparation of molecularly imprinted polymers and its application in a biomimetic immunoassay for the detection of protein
复制标题
表面活性剂介导的溶胶-凝胶法制备分子印迹聚合物及其在仿生免疫分析中检测蛋白质的应用
DOI：
10.1016/j.jpba.2020.113511
复制
发表时间：
2020-10-25
影响因子：
3.4
通讯作者：
Fu Qiang
中科院分区：
文献类型：
--
作者：
Chen Guoning;Shu Hua;Fu Qiang
分子印迹聚合物作为生物抗体的替代品在免疫分析中显示出巨大的潜力。然而，由于蛋白质分子结构的复杂性和有机溶剂的不稳定性，制备蛋白质分子印迹聚合物仍然是一个巨大的挑战。此外，分子印迹聚合物中的非特异性结合位点降低了其作为免疫分析抗体替代物的可行性。本文设计了一种在水溶液中以表面活性剂为介质的溶胶-凝胶体系制备蛋白质分子印迹聚合物。采用封闭策略降低了非特异性交叉反应性，提高了选择性。采用红外光谱、扫描电镜、透射电镜、X射线光电子能谱、振动样品磁强计等手段对产物进行了表征。所制备的分子印迹聚合物对靶分子具有良好的特异性识别，并建立了仿生免疫分析方法。该方法对人血清白蛋白在1-100 μ g/mL（-1）浓度范围内具有良好的线性响应。该方法的检出限为0.3 μ g mL（-1）（3s/K），回收率为85.4-104.5%。本研究表明，采用表面活性剂介导的溶胶-凝胶法制备的分子印迹聚合物具有高选择性，在仿生免疫分析中具有很大的替代抗体的潜力。(C)2020爱思唯尔B. V.保留所有权利。
Molecularly imprinted polymers have demonstrated tremendous potential in the immunoassay as alternatives to biological antibodies. However, the production of molecularly imprinted polymers for protein remains great challenges because of structural complexity and organic solvent instability. In addition, non-specific binding sites in the molecularly imprinted polymers debase the feasibility of it as alternative to antibodies for immunoassay. Here, a surfactant-mediated sol-gel system in an aqueous environment was designed to produce the molecularly imprinted polymers for protein. A blocked strategy was introduced to decrease non-specific cross-reactivity and to improve the selectivity. The developed products were characterized by infrared spectroscopy, scanning electron microscope, transmission electron microscope, X-ray photoelectron spectroscopy, and vibrating sample magnetometer, respectively. The obtained molecularly imprinted polymers exhibited desirable specific recognition towards the target and a biomimetic immunoassay method was developed. The method exhibited a good linear response to human serum albumin in a concentration range of 1-100 mu g mL(-1). The limit of detection of this method was 0.3 mu g mL(-1) (3s/K), and good recoveries ranging from 85.4-104.5% were achieved. This study demonstrated that the molecularly imprinted polymers prepared by the surfactant-mediated sol-gel method can produce high selectivity materials, which had great potential to replace antibodies in a biomimetic immunoassay. (C) 2020 Elsevier B.V. All rights reserved.