Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies
Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies
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DOI：
10.1115/1.2165689
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发表时间：
2006-04-01
影响因子：
1.7
通讯作者：
Dong, C
中科院分区：
文献类型：
--
作者：
Leyton-Mange, J;Yang, S;Dong, C
模拟微毛细管中的流动条件的实验模型已经表明，局部剪切应力和剪切速率可以介导肿瘤细胞和白细胞在内皮上的停滞以及随后的持续粘附。但是进一步的调查已经进行。由于缺乏允许定量测量贴壁细胞上的流体动力学环境的实验模型而受到限制。本研究的目的是开发一种能够获得贴壁细胞上的定量流动曲线的系统。通过结合侧视成像和粒子图像测速（PIV）技术，在体外模型的构建，是能够获得定量的流动数据的细胞粘附到内皮。测量粘附的白细胞上的速度，并计算低和高上游壁剪切下的剪切速率。使用计算流体动力学（CFD）对微毛细管通道进行建模，并将低和高剪切速率下的细胞上的计算的速度分布与实验结果进行比较。然后计算流体施加到每个单元的拖曳力。该系统通过表征粘附细胞上的局部流体动力条件，为未来研究粘附力提供了一种手段。
Experimental models that mimic the flow conditions in microcapillaries have suggested that the local shear stresses and shear rates can mediate tumor cell and leukocyte arrest on the endothelium and subsequent sustained adhesion. However further investigation has been. limited by the lack of experimental models that allow quantitative measurement of the hydrodynamic environment over adherent cells. The purpose of this study was to develop a system capable of acquiring quantitative flow profiles over adherent cells. By combining the techniques of side-view imaging and particle image velocimetry (PIV), an in vitro model was constructed that is capable of obtaining quantitative flow data over cells adhering to the endothelium. The velocity over an adherent leukocyte was measured and the shear rate was calculated under low and high upstream wall shear The microcapillary channel was modeled using computational fluid dynamics (CFD) and the calculated velocity profiles over cells under the low and high shear rates were compared to experimental results. The drag force applied to each cell by the fluid was then computed. This system provides a means for future study of the forces underlying adhesion by permitting characterization of the local hydrodynamic conditions over adherent cells.