Subcortical cytoskeleton periodicity throughout the nervous system.
Subcortical cytoskeleton periodicity throughout the nervous system.
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DOI：
10.1038/srep22741
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发表时间：
2016-03-07
影响因子：
4.6
通讯作者：
Hell SW
中科院分区：
文献类型：
--
作者：
D'Este E;Kamin D;Velte C;Göttfert F;Simons M;Hell SW
超分辨荧光显微镜最近揭示了一个约190 nm的周期性细胞骨架晶格的肌动蛋白，血影蛋白，和其他蛋白质的培养海马神经元膜下。周期性细胞骨架晶格是否是所有神经元的结构特征，以及当轴突被髓鞘形成的神经胶质细胞包裹时，它是如何被修改的还不清楚。在这里，STED纳米显微镜用于证明这种结构是体外几乎所有神经元类型的常见结构。为了检查皮层下的网状结构在髓鞘形成过程中是如何改变的，我们研究了成年小鼠的坐骨神经纤维。肌动蛋白和血影蛋白的周期性在节间被发现，表明无髓鞘和有髓鞘轴突之间没有实质性差异。值得注意的是，肌动蛋白/血影蛋白模式也检测到神经胶质细胞，如培养的少突胶质细胞前体细胞。总之，我们的工作表明，周期性皮层下细胞骨架网络是神经系统细胞的基本特征，而不是以前认为的神经元的独特特征。
Superresolution fluorescence microscopy recently revealed a ~190 nm periodic cytoskeleton lattice consisting of actin, spectrin, and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is a structural feature of all neurons and how it is modified when axons are ensheathed by myelin forming glial cells is not known. Here, STED nanoscopy is used to demonstrate that this structure is a commonplace of virtually all neuron types in vitro. To check how the subcortical meshwork is modified during myelination, we studied sciatic nerve fibers from adult mice. Periodicity of both actin and spectrin was uncovered at the internodes, indicating no substantial differences between unmyelinated and myelinated axons. Remarkably, the actin/spectrin pattern was also detected in glial cells such as cultured oligodendrocyte precursor cells. Altogether our work shows that the periodic subcortical cytoskeletal meshwork is a fundamental characteristic of cells in the nervous system and is not a distinctive feature of neurons, as previously thought.