SELECTIVE BINDING AND TRANSCYTOSIS OF ULEX-EUROPAEUS-1 LECTIN BY MOUSE PEYERS-PATCH M-CELLS IN-VIVO
SELECTIVE BINDING AND TRANSCYTOSIS OF ULEX-EUROPAEUS-1 LECTIN BY MOUSE PEYERS-PATCH M-CELLS IN-VIVO
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DOI：
10.1007/s004410050496
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发表时间：
1995-12-01
影响因子：
3.6
通讯作者：
HIRST, BH
中科院分区：
文献类型：
--
作者：
CLARK, MA;JEPSON, MA;HIRST, BH
采用免疫荧光和电镜技术，在小鼠Peyer’s patch肠环模型中研究了凝集素欧洲巨噬菌凝集素1与小鼠Peyer’s patch滤泡相关上皮细胞的体内相互作用。凝集素靶向小鼠Peyer’s patch m细胞，并迅速内吞和转胞。这些过程伴随着m细胞微绒毛的形态变化和聚合肌动蛋白的重新分配。肠道m细胞在体内对凝集素的选择性结合和摄取表明，m细胞特异性表面糖缀合物可能作为微生物选择性粘附/摄取的受体。
The in vivo interaction of the lectin Ulex europaeus agglutinin 1 with mouse Peyer's patch follicle-associated epithelial cells was studied in the mouse Peyer's patch gut loop model by immunofluorescence and electron microscopy. The lectin targets to mouse Peyer's patch M-cells and is rapidly endocytosed and transcytosed. These processes are accompanied by morphological changes in the M-cell microvilli and by redistribution of polymerised actin. The demonstration of selective binding and uptake of a lectin by intestinal M-cells in vivo suggests that M-cell-specific surface glycoconjugates might act as receptors for the selective adhesion/uptake of microorganisms.