Overexpression of E2F-1 inhibits progression of gastric cancer in vitro
Overexpression of E2F-1 inhibits progression of gastric cancer in vitro
复制标题
E2F-1过表达抑制体外胃癌进展
DOI：
10.1016/j.cellbi.2009.02.015
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发表时间：
2009-06-01
影响因子：
3.9
通讯作者：
Xiao, Qiang
中科院分区：
文献类型：
--
作者：
Xie, Yubo;Wang, Changqing;Xiao, Qiang
E2F-1 在细胞周期调节和细胞内其他生物过程中发挥着关键作用。 E2F-1 在许多组织类型中介导细胞凋亡并抑制肿瘤发生，但关于 E2F-1 表达及其与胃癌肿瘤动力学关系的数据很少。为了更好地了解E2F-1在胃癌生物学特征中的作用，我们研究了E2F-1过表达对胃癌细胞进展的影响。建立了稳定过表达E2F-1的胃癌细胞系（MGC-803/E2F-1）。通过测量细胞存活、集落形成和细胞周期进展来评估 E2F-1 过表达对体外细胞生长的影响。结果清楚地表明，E2F-1 的过表达显着抑制细胞生长和增殖，阻止进入细胞周期的 S 期。 MGC-803/E2F-1 细胞的凋亡率也高于对照细胞。此外，E2F-1降低了胃癌细胞的运动性和侵袭性。 (c) 2009 年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
E2F-1 plays a critical role in cell cycle regulation and other biological processes in cells. E2F-1 mediates apoptosis and suppresses tumorigenesis in many tissue types, but there are few data available on E2F-1 expression and its relationship to tumor kinetics in gastric cancer. To gain better insight into the involvement of E2F-1 in the biological characteristics of gastric tumors, we investigated the effect of E2F-1 overexpression on the progression of gastric carcinoma cells. A gastric cancer cell line stably overexpressing E2F-1 (MGC-803/E2F-1) was established. The influence of E2F-1 overexpression on in vitro cell growth was assessed by measuring cell survival, colony formation, and cell cycle progression. The results clearly show that overexpression of E2F-1 significantly inhibits cell growth and proliferation, blocking entry into the S-phase of the cell cycle. MGC-803/E2F-1 cells also had a higher apoptotic rate than control cells. In addition, E2F-1 reduced the motility and invasion of gastric cancer cells. (c) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.