Expression and function of dopamine in odontoblasts
Expression and function of dopamine in odontoblasts
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DOI：
10.1002/jcp.29314
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发表时间：
2019-10-15
影响因子：
5.6
通讯作者：
Maeda, Hidefumi
中科院分区：
文献类型：
--
作者：
Fujino, Shoko;Hamano, Sayuri;Maeda, Hidefumi
多巴胺(DA)是由酪氨酸羟化酶(TH)催化酪氨酸合成的。最近的一项研究报道，DA促进了小鼠前成骨细胞的矿化。然而，DA在成牙本质细胞中的作用尚未被研究。因此，本实验研究了TH和DA在成牙本质细胞中的表达及DA对成牙本质细胞分化的影响。免疫组织化学染色显示TH和DA在大鼠切牙和磨牙的成牙本质细胞和前成牙本质细胞中有较强的表达。KN-3细胞表达D1样和D2样DA受体。此外，DA促进KN-3细胞向成牙本质细胞分化，而D1样受体拮抗剂和PKA信号阻断剂则抑制这种分化。然而，D2样受体的拮抗剂促进了分化。这些结果提示，前成牙本质细胞和成牙本质细胞中的DA可能通过D1样受体促进成牙本质细胞分化，而不是通过D2样受体和PKA信号以自分泌或旁分泌的方式促进成牙本质细胞的分化，并在牙本质形成中发挥作用。
Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.