Epstein-Barr virus RNA. VI. Viral RNA in restringently and abortively infected Raji cells

Epstein-Barr virus RNA. VI. Viral RNA in restringently and abortively infected Raji cells
复制标题

EB 病毒 RNA。

DOI：
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发表时间：
1981
期刊：
Journal of Virology
影响因子：
5.4
通讯作者：
E. Kieff
E. Kieff
中科院分区：
医学2区
文献类型：
--
作者：
W. King;V. V. van Santen;E. Kieff

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Raji细胞的核RNA和聚腺苷化RNA片段编码的Epstein-Barr病毒DNA片段（分别为35%和18%）比编码的多核糖体RNA片段（10%）要大。多核糖体RNA编码DNA定位在0.05 × 10(8)至0.29 × 10(8), 0.63 × 10(8)至0.66 × 10(8), 1.10 × 10(8)至0.03 × 10(8)道尔顿。由EcoRI片段J (0.05 × 10（8)至0.07 × 10(8)道尔顿）编码的大量小的（160个碱基）非聚腺苷化RNA也存在于Raji细胞的细胞质中。在Raji细胞中诱导早期抗原后，病毒多腺苷化和多核糖体rna的复杂性大幅增加。因此，40%的Epstein-Barr病毒DNA编码核RNA，至少30%的Epstein-Barr病毒DNA编码聚腺苷化和多核糖体RNA。Raji Polyribosomal RNA诱导的细胞被巴尔病毒DNA编码映射为0.05 X 10(8)到0.29 X 10 (8), 0.63 X 10(8)到0.66 X 10(8)和1.10 X 10(8)到0.03 X 10(8)道尔顿和DNA映射eb病毒DNA长独特的区域内的0.39 X 10 (8) 0.49 X 10 (8), 0.51 X 10(8)到0.59 X 10 (8), 0.66 X 10(8)到0.77 X 10(8)和1.02 X 10(8)到1.05 X 10(8)道尔顿。
Nuclear and polyadenylated RNA fractions of Raji cells are encoded by larger fractions of Epstein-Barr virus DNA (35 and 18%, respectively) than encode polyribosomal RNA (10%). Polyribosomal RNA is encoded by DNA mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons. An abundant, small (160-base), non-polyadenylated RNA encoded by EcoRI fragment J (0.05 X 10(8) to 0.07 X 10(8) daltons) is also present in the cytoplasm of Raji cells. After induction of early antigen in Raji cells, there was a substantial increase in the complexity of viral polyadenylated and polyribosomal RNAs. Thus, nuclear RNA was encoded by 40% of Epstein-Barr virus DNA, and polyadenylated and polyribosomal RNAs were encoded by at least 30% of Epstein-Barr virus DNA. Polyribosomal RNA from induced Raji cells was encoded by Epstein-Barr virus DNAs mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons and also by DNAs mapping within the long unique regions of Epstein-Barr virus DNA at 0.39 X 10(8) to 0.49 X 10(8), 0.51 X 10(8) to 0.59 X 10(8), 0.66 X 10(8) to 0.77 X 10(8), and 1.02 X 10(8) to 1.05 X 10(8) daltons.