EVALUATION OF 2, 3, 5-TRIPHENYLTETRAZOLIUM CHLORIDE AS A STAIN FOR DETECTION AND QUANTIFICATION OF EXPERIMENTAL CEREBRAL INFARCTION IN RATS
EVALUATION OF 2, 3, 5-TRIPHENYLTETRAZOLIUM CHLORIDE AS A STAIN FOR DETECTION AND QUANTIFICATION OF EXPERIMENTAL CEREBRAL INFARCTION IN RATS
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DOI：
10.1161/01.str.17.6.1304
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发表时间：
1986-11-01
期刊：
影响因子：
8.3
通讯作者：
BARTKOWSKI, HM
中科院分区：
文献类型：
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作者：
BEDERSON, JB;PITTS, LH;BARTKOWSKI, HM
我们评估了使用 2,3,5-三苯基氯化四唑 (TTC) 作为组织病理学染色剂来识别梗塞大鼠脑组织。对35只正常成年大鼠的大脑中动脉（MCA）进行手术闭塞。在手术封堵后的不同时间，处死大鼠并获得脑切片并用TCC或苏木精和伊红（H和E）染色；对每种方法染色的梗塞组织面积的大小进行量化。在MCA闭塞后24小时处死的大鼠中，梗塞面积的大小为21±。 TTC 的冠状部分的 2%，以及 21 .+-。 H 和 E 为 2%（平均值 .+-.S.D.，N = 13）。通过配对 t 检验，两种染色方法确定的梗死面积大小没有显着差异，并且通过线性回归分析发现每种方法确定的大小之间存在显着相关性（r = 0.91，斜率 = 0.89，y 截距 = 4.4%）。 TTC 染色是一种快速、方便、廉价且可靠的方法，用于在缺血发作 24 小时后检测和定量大鼠脑梗塞。
We have evaluated the use of 2,3,5-triphenyltetrazolium chloride (TTC) as an histopathologic stain for identification of infarcted rat brain tissue. The middle cerebral artery (MCA) of 35 normal adult rats was occluded surgically. At various times after surgical occlusion, rats were sacrificed and brain slices were obtained and stained with TCC or hematoxolin and eosin (H and E); the size of the area of infarcted tissue stained by each method was quantified. In rats sacrificed 24 hr after occlusion of the MCA, the size of the area of infarction was 21 .+-. 2% of the coronal section for TTC, and 21 .+-. 2% for H and E (mean .+-. S.D., N = 13). The size of areas of infarction determined by either staining method was not significantly different in area by the paired t test, and a significant correlation bewteen sizes determined by each method was found by linear regression analysis (r = 0.91, slope = 0.89, and the y intercept = 4.4%). Staining with TTC is a rapid, convenient, inexpensive, and reliable method for the detection and quantification of cerebral infarction in rats 24 hr after the onset of ischemia.