Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA.
Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA.
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DOI：
10.1155/2013/146320
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发表时间：
2013
影响因子：
3.1
通讯作者：
Lam KL
中科院分区：
文献类型：
--
作者：
Katas H;Raja MA;Lam KL
壳聚糖纳米粒作为药物载体具有良好的物理化学性质。本研究的目的是确定制备参数对CS NPs的物理特性和胶体稳定性的调制。在测定其储存稳定性之前，通过与硫酸葡聚糖（DS）的离子相互作用制备CS NP。当CS和DS在pH 4下混合且DS：CS质量比为0.5：1时，产生具有+56.2 ± 1.5 mV表面电荷的353 ± 23 nm的最小CS NP。当BSA/siRNA负载到纳米粒中时，实现了98%的包封率。结果还显示，当在4°C下储存时，CS NP的粒度和表面电荷在长达2周内略有变化。随着DS浓度的增加，得到更大的粒径和表面电荷。总之，NP在4°C下保持足够稳定，并且能够携带和保护蛋白质。
Chitosan nanoparticles (CS NPs) exhibit good physicochemical properties as drug delivery systems. The aim of this study is to determine the modulation of preparative parameters on the physical characteristics and colloidal stability of CS NPs. CS NPs were fabricated by ionic interaction with dextran sulphate (DS) prior to determination of their storage stability. The smallest CS NPs of 353 ± 23 nm with a surface charge of +56.2 ± 1.5 mV were produced when CS and DS were mixed at pH 4 and with a DS : CS mass ratio of 0.5 : 1. An entrapment efficiency of 98% was achieved when BSA/siRNA was loaded into the nanoparticles. The results also showed that particle size and surface charge of CS NPs were slightly changed up to 2 weeks when stored at 4°C. Greater particle size and surface charge were obtained with increasing the concentration of DS. In conclusion, NPs were sufficiently stable when kept at 4°C and able to carry and protect protein.