Role of unc5b in retinal neovascularization in mice with oxygen-induced retinopathy.
Role of unc5b in retinal neovascularization in mice with oxygen-induced retinopathy.
复制标题
unc5b 在氧诱导视网膜病变小鼠视网膜新生血管形成中的作用。
DOI：
10.3980/j.issn.2222-3959.2011.02.05
复制
发表时间：
2011
影响因子：
1.4
通讯作者：
L. Shang
中科院分区：
文献类型：
--
作者：
Dan Liu;X. Xia;Xue;Xiao;L. Shang
目的
探讨unc 5 b在小鼠氧诱导视网膜病变（OIR）视网膜新生血管形成中的作用。
方法
在出生后7（P7），将C57 BL/6 J小鼠暴露于75%±2%的氧气中5天。在出生后12（P12），将小鼠带回室内空气（21%氧气）以诱导视网膜新生血管形成。进行蛋白质印迹分析以检查unc 5 b在小鼠视网膜中的时间表达。采用unc 5 b和同种凝集素B4的双重染色来确定unc 5 b在小鼠视网膜中的位置。通过玻璃体内注射unc 5 b-FC评价unc 5 b对OIR小鼠视网膜新生血管的影响。通过计数内界膜上方的新生血管细胞核和通过用荧光素葡聚糖灌注的平板视网膜的血管造影来测量视网膜新生血管。
结果
与年龄匹配的正常小鼠相比，在P17和P21，unc 5 b在OIR小鼠视网膜中的表达显著增加。Unc 5 b在OIR视网膜血管中表达明显，在正常视网膜血管中表达阴性。注射unc 5 b-FC的眼睛中的视网膜新血管形成显著减少。
结论
Unc 5 b-FC能有效抑制OIR诱导的视网膜新生血管形成，有望成为治疗缺血性视网膜疾病的一种新的有效药物。
AIM
To explore the role of unc5b in retinal neovascularization in murine oxygen-induced retinopathy (OIR).
METHODS
On postnatal 7(P7), C57BL/6J mice were exposed to 75%±2% oxygen for 5 days. On postnatal 12(P12), the mice were brought back to the room air (21% oxygen) to induce retinal neovascularization. Western blot analysis was performed to examine the temporal expression of unc5b in murine retinas. Double staining for unc5b and isolectin B4 were employed to determine the location of unc5b in murine retinas. The effect of unc5b on retinal neovascularization was evaluated by intravitreal injection of unc5b-FC in mice with OIR. Retinal neovascularization was measured by counting neovascular cell nuclei above the internal limiting membrane and by angiography of flat-mounted retinas perfused with fluorescein dextran.
RESULTS
Compared to age-matched normal mice, the expression of unc5b was significantly increased in retinas of OIR mice on P17 and P21. Unc5b was apparently expressed in retinal vessels of OIR while being negative in normal retinal vessels. Retinal neovascularization in eyes injected with unc5b-FC was significantly reduced.
CONCLUSION
Unc5b-FC can effectively inhibit retinal neovascularization induced by OIR. It may serve as a powerful and novel therapy for ischemia-induced retinal disease.
影响因子：
20.1
作者：
Epting, Daniel;Wendik, Bjoern;Kroll, Jens
通讯作者：
Kroll, Jens