DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain
DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain
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小鼠 Dlk1-Dio3 结构域中母源甲基化 DMR 的 DNA 甲基化动态
DOI：
10.1016/j.febslet.2014.10.038
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发表时间：
2014-12-20
期刊：
影响因子：
3.5
通讯作者：
Wu, Qiong
中科院分区：
文献类型：
--
作者：
Zeng, Tie-Bo;He, Hong-Juan;Wu, Qiong
小鼠δ样同源物1和III型碘甲状腺原氨酸脱碘酶（Dlk 1-Dio 3）印迹结构域包含三个已知的父系甲基化差异甲基化区域（DMR）：基因间DMR（IG-DMR）、母系表达的3-DMR（Gtl 2-DMR）和Dlk 1-DMR。在这里，我们报告了第一个母体甲基化的DMR，CpG岛2（CGI-2），位于miR-1188下游约800 bp处。CGI-2在精子和卵母细胞中高度甲基化，在植入前胚胎中去甲基化，并在植入后发育过程中差异性地重新甲基化。CGI-2与Gtl 2-DMR和Dlk 1-DMR类似，在胚胎第7.5天（E7.5）之前获得差异甲基化。H3 K4 me 3和H3 K9 me 3组蛋白修饰都在CGI-2富集。此外，CCCTC结合因子（CTCF）在体内结合CGI-2的两个等位基因。这些结果有助于在这一领域的印迹调控的调查。(C)2014年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The mouse delta-like homolog 1 and type III iodothyronine deiodinase (Dlk1-Dio3) imprinted domain contains three known paternally methylated differentially methylated regions (DMRs): intergenic DMR (IG-DMR), maternally expressed 3-DMR (Gtl2-DMR), and Dlk1-DMR. Here, we report the first maternally methylated DMR, CpG island 2 (CGI-2), is located approximately 800 bp downstream of miR-1188. CGI-2 is highly methylated in sperm and oocytes, de-methylated in pre-implantation embryos, and differentially re-methylated during post-implantation development. CGI-2, similarly to Gtl2-DMR and Dlk1-DMR, acquires differential methylation prior to embryonic day 7.5 (E7.5). Both H3K4me3 and H3K9me3 histone modifications are enriched at CGI-2. Furthermore, CCCTC-binding factor (CTCF) binds to both alleles of CGI-2 in vivo. These results contribute to the investigation of imprinting regulation in this domain. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.