Identification of an Arabidopsis thaliana protein that binds to tomato mosaic virus genomic RNA and inhibits its multiplication
Identification of an Arabidopsis thaliana protein that binds to tomato mosaic virus genomic RNA and inhibits its multiplication
复制标题
DOI：
10.1016/j.virol.2008.07.033
复制
发表时间：
2008-10-25
期刊：
影响因子：
3.7
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Fujisaki, Koki;Ishikawa, Masayuki
正链RNA病毒的基因组RNA携带RNA元件，这些RNA元件通过与病毒和细胞蛋白相互作用在病毒增殖中发挥积极作用，或者在某些情况下发挥消极作用。在本研究中，我们纯化了与番茄花叶病毒（ToMV）基因组RNA的5'或3'末端区域特异性结合的拟南芥蛋白，这些蛋白包含翻译和RNA复制的重要调控元件，并通过质谱分析鉴定了这些蛋白。这些宿主蛋白之一，命名为BTR 1，具有三个异质核核糖核蛋白K同源RNA结合结构域，并优先结合到RNA片段，该片段包含130K和180K复制蛋白基因的起始密码子周围的序列。BTR 1的敲除和过表达分别特异性地增强和抑制ToMV在接种的A.而在原生质体中几乎检测不到这种效应。这些结果表明，BTR1负调控ToMV的局部传播。(C)2008年爱思唯尔公司All rights reserved.
The genomic RNAs of positive-strand RNA viruses carry RNA elements that play positive, or in some cases, negative roles in virus multiplication by interacting with viral and cellular proteins. In this study, we purified Arabidopsis thaliana proteins that specifically bind to 5' or 3' terminal regions of tomato mosaic virus (ToMV) genomic RNA, which contain important regulatory elements for translation and RNA replication, and identified these Proteins by mass spectrometry analyses. One of these host proteins, named BTR1, harbored three heterogeneous nuclear ribonucleoprotein K-homology RNA-binding domains and preferentially bound to RNA fragments that contained a sequence around the initiation codon of the 130K and 180K replication protein genes. The knockout and overexpression of BTR1 specifically enhanced and inhibited, respectively, ToMV multiplication in inoculated A. thaliana leaves, while such effect was hardly detectable in protoplasts. These results suggest that BTR1 negatively regulates the local spread of ToMV. (C) 2008 Elsevier Inc. All rights reserved.