Ventricular function during exercise in mice and rats
Ventricular function during exercise in mice and rats
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DOI：
10.1152/ajpregu.00340.2011
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
DiCarlo, Stephen E.
中科院分区：
文献类型：
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作者：
Lujan, Heidi L.;Janbaih, Hussein;DiCarlo, Stephen E.
Lujan HL，Janbaih H，Feng HZ，Jin JP，DiCarlo SE.小鼠和大鼠运动时的心室功能。Am J Physiol Regul Integr Comp Physiol 302：R68-R74，2012.首次发表于2011年10月19日; doi：10.1152/ajpregu.00340.2011。与其他实验模型相比，小鼠在左心室（LV）功能的分子研究中具有许多优势。因此，人们对用于测定心脏功能的有意识的、长期装有仪器的、自由移动的小鼠模型有着浓厚的兴趣和强烈的需求。为了满足这一需求，我们使用遥测设备重复测量清醒小鼠在休息和运动时的LV功能。作为参考，我们比较了小鼠的反应与相同仪器清醒大鼠的反应。遥测装置（大鼠PA-C40;小鼠PA-C10; Data Sciences International，圣保罗，MN）的发射器主体通过腹侧入路（大鼠）或皮下左胁腹（小鼠）置于腹膜内空间。将位于导管尖端内的压力传感器通过心尖刺伤（大鼠为18 gauge;小鼠为21 gauge）插入左心室，以连续、非栓系地记录脉动LV压力。允许动物至少恢复1周，并恢复其术前体重。在恢复期，对动物进行处理、称重，并使其适应实验室、跑步机和研究者。随后，记录静息和分级运动试验期间的LV参数。该结果首次记录了清醒小鼠和大鼠运动期间心室功能的系列评估。这种方法可能被用于推进推动心血管研究的概念和想法。
Lujan HL, Janbaih H, Feng HZ, Jin JP, DiCarlo SE. Ventricular function during exercise in mice and rats. Am J Physiol Regul Integr Comp Physiol 302: R68-R74, 2012. First published October 19, 2011; doi:10.1152/ajpregu.00340.2011.-The mouse has many advantages over other experimental models for the molecular investigation of left ventricular (LV) function. Accordingly, there is a keen interest in, as well as an intense need for, a conscious, chronically instrumented, freely moving mouse model for the determination of cardiac function. To address this need, we used a telemetry device for repeated measurements of LV function in conscious mice at rest and during exercise. For reference, we compared the responses in mice to the responses in identically instrumented conscious rats. The transmitter body of the telemetry device (rat PA-C40; mouse PA-C10; Data Sciences International, St. Paul, MN) was placed in the intraperitoneal space through a ventral abdominal approach (rat) or subcutaneously on the left flank (mouse). The pressure sensor, located within the tip of a catheter, was inserted into the left ventricle through an apical stab wound (18 gauge for rat; 21 gauge for mouse) for continuous, nontethered, recordings of pulsatile LV pressure. A minimum of 1 wk was allowed for recovery and for the animals to regain their presurgical weight. During the recovery period, the animals were handled, weighed, and acclimatized to the laboratory, treadmill, and investigators. Subsequently, LV parameters were recorded at rest and during a graded exercise test. The results document, for the first time, serial assessment of ventricular function during exercise in conscious mice and rats. This methodology may be adopted for advancing the concepts and ideas that drive cardiovascular research.