Increases of CD80 and CD86 Expression on Peripheral Blood Cells and their Gene Polymorphisms in Autoimmune Thyroid Disease
Increases of CD80 and CD86 Expression on Peripheral Blood Cells and their Gene Polymorphisms in Autoimmune Thyroid Disease
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DOI：
10.1080/08820139.2019.1688343
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发表时间：
2019-11
影响因子：
2.8
通讯作者：
A. Watanabe;N. Inoue;Mikio Watanabe;Mayu Yamamoto;Haruka Ozaki;Y. Hidaka;Y. Iwatani
中科院分区：
文献类型：
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作者：
A. Watanabe;N. Inoue;Mikio Watanabe;Mayu Yamamoto;Haruka Ozaki;Y. Hidaka;Y. Iwatani
摘要 自身免疫性甲状腺疾病（AITD）的预后很难预测，例如格雷夫斯病（GD）和桥本病（HD）。 CD80和CD86共刺激信号与T细胞受体信号协同促进T细胞活化。为了阐明 CD80 和 CD86 与 AITD 发病机制之间是否存在关联，我们检查了 CD80 和 CD86 的表达和基因多态性。采用流式细胞仪检测外周血细胞上CD80和CD86蛋白的表达，并通过PCR-RFLP和Taqman PCR方法对CD80和CD86基因多态性进行基因分型。在对 B 淋巴细胞的分析中，发现患者中 CD80+ 细胞升高 (>8%) 的情况比对照受试者更常见，而且难治性 GD 患者比缓解期 GD 患者更常见 (p= .0176)。 GD 和 HD 患者单核细胞上 CD86 表达的平均荧光强度高于对照受试者（分别为 p= <0.0001 和 p= .0017）。 CD80 rs1599795 T 等位基因携带者在重度 HD 患者中比在轻度 HD 患者中更常见。 CD86 rs2715267 AA 基因型在 HD 患者中比对照组更常见。结论 AITD患者外周血中B细胞CD80和单核细胞CD86表达升高,尤其是重症患者,其基因多态性与HD的易感性和严重程度相关。
ABSTRACT The prognosis of autoimmune thyroid diseases (AITDs), such as Graves’ disease (GD) and Hashimoto’s disease (HD), are difficult to predict. Both CD80 and CD86 costimulatory signals promote T cell activation in cooperation with T cell receptor signal. To clarify whether any association between CD80 and CD86 and the pathogenesis of AITD exist, we examined the expressions and gene polymorphisms of CD80 and CD86. We examined the expressions of CD80 and CD86 proteins on peripheral blood cells by flowcytometry and genotyped CD80 and CD86 gene polymorphisms by PCR-RFLP and Taqman PCR methods. In the analysis of the Blymphocytes elevated CD80+ cells (>8%) were found more often in the patients than in control subjects, and also it was more frequent in patients with intractable GD than in those with GD in remission (p= .0176). The mean fluorescence intensity of CD86 expression on monocytes was higher in GD and HD patients than in control subjects (p= <0.0001 and p= .0017, respectively). CD80 rs1599795 T allele carriers were more frequent in patients with severe HD than in those with mild HD. CD86 rs2715267 AA genotype was more frequent in HD patients than in controls. In conclusion, the expressions of CD80 on Bcells and of CD86 on monocytes were increased in peripheral blood from patients with AITD, especially in severe cases, and their gene polymorphisms are associated with the susceptibility and the severity of HD.