Determining the antibiotic resistance potential of the indigenous oral microbiota of humans using a metagenomic approach
Determining the antibiotic resistance potential of the indigenous oral microbiota of humans using a metagenomic approach
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DOI：
10.1111/j.1574-6968.2006.00221.x
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发表时间：
2006-05-01
影响因子：
2.1
通讯作者：
Wilson, M
中科院分区：
文献类型：
--
作者：
Diaz-Torres, ML;Villedieu, A;Wilson, M
对微生物群落中编码抗生素耐药性的基因的流行率和特性的研究通常只在该群落中可培养的成员身上进行。然而，通过对这类研究采用元基因组学方法，有可能包括目前尚未培养的生物体。在这项研究中，从三组20名成年人类中制备了四个口腔微生物区系的元基因组文库，并筛选了具有抗生素抗性的克隆。四个文库中均存在四环素和阿莫西林耐药克隆，其中三个文库中存在庆大霉素耐药克隆。经鉴定，克隆的四环素抗性基因分别为tet(M)、tet(O)、tet(Q)、tet(W)、tet(37)和tet(A)。然而，在所有三组被调查的个人中，只检测到了前三种情况。
Studies of the prevalence and identity of genes encoding resistance to antibiotics in a microbial community are usually carried out on only the cultivable members of the community. However, it is possible to include the as-yet-uncultivable organisms present by adopting a metagenomic approach to such studies. In this investigation, four metagenomic libraries of the oral microbiota were prepared from three groups of 20 adult humans and screened for antibiotic-resistant clones. Clones resistant to tetracycline and amoxycillin were present in all four libraries while gentamicin-resistant clones were found in three of the libraries. The genes encoding tetracycline resistance in the clones were identified and found to be tet(M), tet(O), tet(Q), tet(W), tet37 and tet(A). However, only the first three of these were detected in all three groups of individuals investigated.