Raman spectroscopy and imaging of β-carotene in live corpus luteum cells
Raman spectroscopy and imaging of β-carotene in live corpus luteum cells
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DOI：
10.1016/s0378-4320(02)00020-9
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发表时间：
2002-06-17
影响因子：
2.2
通讯作者：
Batchelder, DN
中科院分区：
文献类型：
--
作者：
Arikan, S;Sands, HS;Batchelder, DN
拉曼光谱已被用来识别和定位β-胡萝卜素在个别活的黄体细胞。细胞是新鲜制备的或培养的;后者在存在或不存在β-胡萝卜素的情况下以富集的牛高密度脂蛋白的形式孵育。利用几种拉曼光谱和成像技术研究了黄体细胞。这些技术没有给出细胞内β-胡萝卜素的准确浓度水平，但说明了分子的分布。发现新鲜制备的黄体细胞含有相当浓度的β-胡萝卜素。在几天的时间内，浓度逐渐降低到几乎检测不到的水平;在不存在β-胡萝卜素的情况下培养的细胞中发现了类似的结果。对于在β-胡萝卜素存在下培养的细胞，分子浓度维持长达2周。碎片细胞的拉曼光谱表明，β-胡萝卜素主要集中在富含脂质的细胞组分中，在微粒体组分中浓度最高。拉曼成像技术显示，β-胡萝卜素分布在整个体积的黄体细胞与较高的水平发生在不同的网站，包括表面。(C)2002 Elsevier Science B. V.保留所有权利。
Raman spectroscopy has been used to identify and locate beta-carotene within individual living luteal cells. The cells were either freshly prepared or cultured; the latter was incubated in the presence or absence of beta-carotene in the form of enriched bovine high-density lipoprotem. Luteal cells were investigated using several Raman spectroscopic and imaging techniques. These techniques did not give accurate concentration levels of beta-carotene within parts of the cell but illustrated the distribution of the molecule. Freshly prepared luteal cells were found to contain an appreciable concentration of beta-carotene. Over a period of several days, the concentration gradually reduced to a nearly undetectable level; similar results were found for cells cultured in the absence of the beta-carotene. For cells cultured in the presence of beta-carotene, the molecular concentration was maintained for as long as 2 weeks. The Raman spectra of fragmented cells showed that the beta-carotene is predominantly localised in the lipid-rich cell components, with the concentration highest in the microsomal fraction. The Raman imaging techniques revealed that beta-carotene was spread over the entire volume of the luteal cells with higher levels occurring at distinct sites, including the surface. (C) 2002 Elsevier Science B.V. All rights reserved.