An integrated nanoliter DNA analysis device
An integrated nanoliter DNA analysis device
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DOI：
10.1126/science.282.5388.484
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发表时间：
1998-10-16
期刊：
影响因子：
56.9
通讯作者：
Burke, DT
中科院分区：
文献类型：
--
作者：
Burns, MA;Johnson, BN;Burke, DT
开发了一种使用微制造流体通道、加热器、温度传感器和荧光探测器来分析纳米升大小的DNA样本的装置。该装置能够测量含水试剂和含有DNA的溶液，将溶液混合在一起，放大或消化DNA以形成离散产物，并分离和缺陷这些产物。没有外部镜头，加热器，或机械泵是必要的完整的样品处理和分析。因为所有的组件都是使用传统的光刻生产技术制造的，所以它们作为一个单一的封闭系统运行。这些组件有可能以低单位成本组装成复杂的、低功耗的综合分析系统。便携式、可靠的仪器的可用性可能促进DNA分析在诸如快速医疗诊断和使用点农业测试等应用中的使用。
A device was developed that uses microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors to analyze nanoliter-size DNA samples. The device is capable of measuring aqueous reagent and DNA-containing solutions, mixing the solutions together, amplifying or digesting the DNA to form discrete products, and separating and defecting those products. No external Lenses, heaters, or mechanical pumps are necessary for complete sample processing and analysis. Because all of the components are made using conventional photolithographic production techniques, they operate as a single closed system. The components have the potential for assembly into complex, low-power, integrated analysis systems at Low unit cost, The availability of portable, reliable instruments may facilitate the use of DNA analysis in applications such as rapid medical diagnostics and point-of-use agricultural testing.