Expression of the androgen receptor and an androgen-responsive protein, apolipoprotein D, in human breast cancer.
Expression of the androgen receptor and an androgen-responsive protein, apolipoprotein D, in human breast cancer.
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人类乳腺癌中雄激素受体和雄激素响应蛋白载脂蛋白D的表达。
DOI：
10.1038/bjc.1996.513
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发表时间：
1996-10
影响因子：
8.8
通讯作者：
Tilley, W D
中科院分区：
文献类型：
--
作者：
Hall, R E;Aspinall, J O;Horsfall, D J;Birrell, S N;Bentel, J M;Sutherland, R L;Tilley, W D
关于雄激素受体（AR）在人类乳腺癌中的活性和功能知之甚少。在本研究中，AR在未经治疗的原发性乳腺癌中使用抗血清的受体的氨基和羧基末端和定量使用彩色视频图像分析。对于每种抗血清，观察到组织浓度与AR阳性细胞百分比之间存在强相关性。然而，在个体乳腺癌标本中使用氨基末端和羧基末端AR抗血清的阳性细胞百分比的比较揭示了羧基末端染色不一致地增加的肿瘤子集。这些发现表明在一定比例的乳腺癌细胞中存在氨基末端截短的AR，或者存在影响氨基末端AR抗血清结合的AR突变或相关蛋白质改变。雄激素调节的糖蛋白，载脂蛋白D（apo-D）的免疫组化表达，也在乳腺癌标本进行了评价。在乳腺肿瘤中观察到apo-D染色的局灶性阳性，但并不总是与AR阳性细胞共定位。此外，乳腺癌标本中AR和apo-D染色阳性细胞百分比之间无明显相关性。这些结果表明，虽然载脂蛋白D的表达是雄激素调节的人乳腺癌细胞系在体外，其在原发性乳腺癌的表达可能是由其他因素。AR在原发性乳腺癌中的表达也表明该受体可能参与肿瘤反应或对内分泌治疗的异常反应。
Little is known regarding the activity and function of the androgen receptor (AR) in human breast cancer. In the present study AR was evaluated in untreated primary breast cancers using antisera to the amino- and carboxy-termini of the receptor and quantitated using colour video image analysis. A strong correlation between tissue concentration and percentage AR-positive cells was observed for each antiserum. However, comparison of percentage positive cells using the amino- and carboxy-terminal AR antisera in individual breast cancer specimens revealed a subset of tumours with discordantly increased staining for the carboxy terminus. These findings suggest the presence of amino-terminal-truncated AR in a proportion of breast cancer cells or presence of AR mutations or associated protein alterations that affect binding of the amino-terminal AR antiserum. Immunohistochemical expression of the androgen-regulated glycoprotein, apolipoprotein D (apo-D), was also evaluated in the breast cancer specimens. Focal positivity of apo-D staining, which did not always co-localise with AR-positive cells, was observed within breast tumours. Furthermore, no correlation was evident between percentage positive cells stained for AR and apo-D in breast cancer specimens. These findings indicate that, although apo-D expression is androgen regulated in human breast cancer cell lines in vitro, its expression in primary breast cancers may be regulated by other factors. The expression of AR in primary breast cancers also suggests that the receptor may be involved in tumour responsiveness or in abnormal responses to endocrine therapies.