Embryonic tissue induces growth of adult axons from myelinated fiber tracts
Embryonic tissue induces growth of adult axons from myelinated fiber tracts
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DOI：
10.1006/exnr.1997.6476
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发表时间：
1997-06-01
影响因子：
5.3
通讯作者：
Raisman, G
中科院分区：
文献类型：
--
作者：
Davies, SJA;Field, PM;Raisman, G
悬浮液的晚期胚胎海马组织进行显微注射，以便完全封闭在有髓纤维束的成年大鼠海马伞。先前的研究表明，来自此类移植神经元的轴突很容易穿过移植物/宿主界面并迅速延伸穿过宿主纤维束。目前的研究表明，来自宿主纤维束的成年轴突也可以以相反的方向穿过该界面并进入移植物。生物素葡聚糖示踪显示，成人宿主菌毛轴突穿过胚胎移植物，并在移植物内形成终末分支。顺行性电子致密变性的电子显微镜检查证实，这些宿主轴突形成突触末端，占移植物神经元中突触的至少6.6%。因此，与胚胎神经组织的接触可以诱导有髓鞘中央束中的成体纤维的伸长生长。(C)1997年学术出版社。
Suspensions of late embryonic hippocampal tissue were microinjected so as to be completely enclosed within the myelinated fiber bundles of the adult rat fimbria. Previous studies have shown that the axons from such transplanted neurons readily cross the graft/host interface and extend rapidly through the host fiber tract. The present study shows that the adult axons from the host fiber tract can also cross this interface in the opposite direction and enter the transplants. Biotin dextran tracing shows that the adult host fimbrial axons traverse the embryonic grafts and also form terminal arborizations within the transplants. Electron microscopy of orthograde electron-dense degeneration confirms that these host axons form synaptic terminals accounting for at least 6.6% of the synapses in the neuropil of the transplant. Thus, contact with embryonic nervous tissue can induce elongative growth by the adult fibers in a myelinated central tract. (C) 1997 Academic Press.