Dietary supplementation of L-carnosine prevents ischemia/reperfusion-induced renal injury in rats.
Dietary supplementation of L-carnosine prevents ischemia/reperfusion-induced renal injury in rats.
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发表时间：
2005-02
影响因子：
2
通讯作者：
T. Fujii;M. Takaoka;N. Tsuruoka;Y. Kiso;Takaharu Tanaka;Y. Matsumura
中科院分区：
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作者：
T. Fujii;M. Takaoka;N. Tsuruoka;Y. Kiso;Takaharu Tanaka;Y. Matsumura
研究了饲粮中添加l-肌肽（β -丙烯酰- l-组氨酸）对大鼠缺血/再灌注性急性肾功能衰竭（ARF）的影响。对侧肾切除术后2周，阻断左肾动静脉45分钟后再灌注诱导缺血性ARF。测定肾功能参数如血尿素氮、血浆肌酐、肌酐清除率、尿流量、尿渗透压和钠排泄分数。ARF大鼠再灌注后1 d肾功能明显下降。预先喂食含左旋肌肽的日粮（0.0001 w/w%） 2周，可减轻缺血/再灌注引起的肾功能障碍。ARF大鼠肾脏组织病理学检查显示肾小管坏死、小管内蛋白样铸型和髓质充血等严重的肾损害，这些损害也被膳食补充l -肌肽显著抑制。这些发现有力地表明，补充l -肌肽在缺血性ARF的发展中是有用的预防性治疗。
The effects of dietary supplementation of L-carnosine (beta-alanyl-L-histidine) on ischemia/reperfusion-induced acute renal failure (ARF) in rats were examined. Ischemic ARF was induced by occlusion of the left renal artery and vein for 45 min followed by reperfusion, 2 weeks after contralateral nephrectomy. Renal functional parameters such as blood urea nitrogen, plasma creatinine, creatinine clearance, urine flow, urinary osmolality and fractional excretion of sodium were measured. Renal function in ARF rats markedly decreased at 1 d after reperfusion. Prior feeding of L-carnosine-containing diet (0.0001 w/w%) for 2 weeks attenuated the ischemia/reperfusion-induced renal dysfunction. Histopathological examination of the kidney of ARF rats revealed severe renal damages, such as tubular necrosis, proteinaceous casts in tubuli and medullary congestion, which were also significantly suppressed by the dietary supplementation of L-carnosine. These findings strongly suggest that L-carnosine supplementation is useful as a prophylactic treatment in the development of the ischemic ARF.