Expression of glutathione S-transferase B1, B2, Mu and Pi in breast cancers and their relationship to oestrogen receptor status.
Expression of glutathione S-transferase B1, B2, Mu and Pi in breast cancers and their relationship to oestrogen receptor status.
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DOI：
10.1038/bjc.1989.375
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发表时间：
1989-12
影响因子：
8.8
通讯作者：
Beckett, G J
中科院分区：
文献类型：
--
作者：
Howie, A F;Miller, W R;Hawkins, R A;Hutchinson, A R;Beckett, G J
用放射免疫分析法测定了28例雌激素受体（ER）丰富和30例ER缺乏的乳腺肿瘤细胞浆中谷胱甘肽S-转移酶（GST）B_1和B_2（α）、Pi和Mu的浓度。GST B1，B2和Pi在所有58个乳腺肿瘤细胞质中检测到，而GST Mu仅在28个中发现。GST中，Pi在所有细胞质中表达最强，ER-贫肿瘤细胞质中的浓度显著高于ER-富肿瘤（P <0.01）。与GST Pi一样，GST B1和GST B2在ER-阴性肿瘤细胞液中的水平最高; GST B1和GST B2的水平呈正相关（r = 0.66，P <0.001）。ER状态和GST Mu之间没有定量或定性相关性，GST Mu在46%的ER丰富和50%的ER贫乏肿瘤细胞质中表达。GST表达与年龄、绝经状态、淋巴结受累或肿瘤T分期之间无相关性。这些数据表明，一个共同的机制是负责GST诱导ER-穷人的肿瘤和无效的Mu表型没有增加的易感性发展乳腺癌。
The concentrations of glutathione S-transferase (GST) B1 and B2 (Alpha), Pi and Mu have been measured by radioimmunoassay in cytosols from 28 oestrogen receptor (ER) rich an 30 ER-poor breast tumours. GST B1, B2 and Pi was detected in all 58 breast tumour cytosols whilst GST Mu was found in only 28. Of the GSTs, Pi was expressed most strongly in all cytosols and the concentration was significantly higher in ER-poor tumour cytosols than in ER-rich tumours (P less than 0.01). As with GST Pi, the highest levels of GST B1 and GST B2 were found in ER-poor tumour cytosols; the levels of GST B1 and GST B2 were positively correlated (r = 0.66, P less than 0.001). No quantitative or qualitative association was found between ER status and GST Mu which was expressed in 46% of ER-rich and 50% of ER-poor tumour cytosols. No relationship could be found between GST expression and age, menopausal status, lymph node involvement or tumour T stage in the subgroup of patients in whom this information was available. These data suggest that a common mechanism is responsible for GST induction in ER-poor tumours and that the nulled Mu phenotype has no increased susceptibility to developing breast cancer.