Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis
Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis
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DOI：
10.1128/jcm.43.4.1995-1997.2005
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发表时间：
2005-04-01
影响因子：
9.4
通讯作者：
Keim, P
中科院分区：
文献类型：
--
作者：
Easterday, WR;Van Ert, MN;Keim, P
围绕plcR基因无意义突变设计的TaqMan-minor凹槽结合试验用于对炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌分离株进行基因分型。该试验将炭疽芽孢杆菌与这些遗传近邻区分开来，并确定无义突变在全球89种遗传多样化的炭疽芽孢杆菌菌株中普遍存在。
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.