CLONING AND SEQUENCING OF LOL PI, THE MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN
CLONING AND SEQUENCING OF LOL PI, THE MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN
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DOI：
10.1016/0014-5793(91)80151-r
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发表时间：
1991-02-25
期刊：
影响因子：
3.5
通讯作者：
KNOX, RB
中科院分区：
文献类型：
--
作者：
GRIFFITH, IJ;SMITH, PM;KNOX, RB
我们已经分离出一个编码主要糖蛋白过敏原Lol pI的全长cDNA克隆。采用cDNA表达文库的免疫筛选和Lol pi特异性转录物的PCR扩增相结合的方法筛选该克隆。在细菌中作为融合蛋白表达的Lol pI被草花粉过敏受试者血清中存在的特异性IgE抗体识别。Northern分析表明，Lol pI转录本仅在黑麦草花粉中表达。Lol pI的分子克隆为研究变应原的结构-功能关系提供了分子遗传学方法。
We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.