Notch Signaling Regulates the Function and Phenotype of Dendritic Cells in Helicobacter pylori Infection.
Notch Signaling Regulates the Function and Phenotype of Dendritic Cells in Helicobacter pylori Infection.
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Notch 信号调节幽门螺杆菌感染中树突状细胞的功能和表型。
DOI：
10.3390/microorganisms11112818
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发表时间：
2023-11-20
期刊：
影响因子：
4.5
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Liu Q;Chen C;He Y;Mai W;Ruan S;Ning Y;Li Y
Notch信号转导调控树突状细胞（DCs）的功能和表型，以及DCs与CD 4 + T细胞之间的相互作用。然而，Notch信号在幽门螺杆菌（H. pylori）感染仍然难以捉摸。小鼠骨髓来源的树突状细胞（BMDC）在H.采用实时荧光定量PCR（qRT-PCR）、Western blot、酶联免疫吸附试验（ELISA）和流式细胞术检测共培养物对pylori的刺激作用、Notch组分、细胞因子和表面标志物的水平以及CD 4 + T细胞的分化。与对照组相比，所有Notch受体和Notch配体Dll 4和Jagged 1的mRNA表达在H.幽门刺激的BMDC。DAPT阻断Notch信号通路可影响H. pylori致敏的BMDCs，Notch 1、Notch 3、Notch 4、Dll 1、Dll 3和Jagged 2的表达降低。此外，DAPT预处理降低了BMDCs成熟标志物CD 80、CD 83、CD 86和主要组织相容性复合物II类（MHC-II）的表达，并进一步使Th 17/Treg平衡向Treg倾斜。Notch信号调节DC的功能和表型，从而介导H.幽门感染
Notch signaling manipulates the function and phenotype of dendritic cells (DCs), as well as the interaction between DCs and CD4+ T cells. However, the role of Notch signaling in Helicobacter pylori (H. pylori) infection remains elusive. Murine bone marrow-derived dendritic cells (BMDCs) were pretreated in the absence or presence of Notch signaling inhibitor DAPT prior to H. pylori stimulation and the levels of Notch components, cytokines and surface markers as well as the differentiation of CD4+ T cells in co-culture were measured using quantitative real-time PCR (qRT-PCR), Western blot, enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Compared with the control, the mRNA expression of all Notch receptors and Notch ligands Dll4 and Jagged1 was up-regulated in H. pylori-stimulated BMDCs. The blockade of Notch signaling by DAPT influenced the production of IL-1β and IL-10 in H. pylori-pulsed BMDCs, and reduced the expression of Notch1, Notch3, Notch4, Dll1, Dll3 and Jagged2. In addition, DAPT pretreatment decreased the expression of maturation markers CD80, CD83, CD86, and major histocompatibility complex class II (MHC-II) of BMDCs, and further skewed Th17/Treg balance toward Treg. Notch signaling regulates the function and phenotype of DCs, thus mediating the differentiation of CD4+ T cells during H. pylori infection.
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影响因子：
7.4
作者：
Ma L;Xue H;Qi R;Wang Y;Yuan L
通讯作者：
Yuan L
影响因子：
15.9
作者：
Ito, Toshihiro;Schaller, Matthew;Kunkel, Steven L.
通讯作者：
Kunkel, Steven L.
影响因子：
6.4
作者：
Lin, Chu-Lun;Huang, Huei-Mei;Lee, Yueh-Lun
通讯作者：
Lee, Yueh-Lun
影响因子：
3.1
作者：
Khamri, Wafa;Walker, Marjorie M.;Lombardi, Giovanna
通讯作者：
Lombardi, Giovanna
影响因子：
3.1
作者：
Kranzer, K;Eckhardt, A;Schneider-Brachert, W
通讯作者：
Schneider-Brachert, W