The First Type III Domain of Fibronectin is Associated with the Expression of Cytokines within the Lung Tumor Microenvironment.
The First Type III Domain of Fibronectin is Associated with the Expression of Cytokines within the Lung Tumor Microenvironment.
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DOI：
10.7150/jca.2.478
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发表时间：
2011
影响因子：
3.9
通讯作者：
McKeown-Longo PJ
中科院分区：
文献类型：
--
作者：
Zheng M;Jones DM;Horzempa C;Prasad A;McKeown-Longo PJ
最近的研究指出，组织力学的变化是恶性肿瘤发展的一个促成因素。组织刚性的增加与细胞外基质中纤维连接蛋白III型结构域的展开有关。这种情况对肺内细胞功能的影响还不是很清楚。在本研究中，我们评价了一种代表纤维连接蛋白第一类型III型重复序列(FnIII-1c)部分未折叠中间产物的多肽对成人肺成纤维细胞炎症基因表达的影响。FnIII-1c诱导肺成纤维细胞表达细胞因子CXCL1-3、IL-8和肿瘤坏死因子-α。IL-8表达的增加依赖于Toll样受体2和核因子κB。肺鳞状细胞癌组织阵列的免疫组织化学显示与肌成纤维细胞共排列的IL-8和纤维连接蛋白纤维广泛染色。这些数据提示了一种模型，在该模型中，继发于肌成纤维细胞产生的张力的FnIII结构域的展开可以诱导存在于肺肿瘤内的间质成纤维细胞释放细胞因子。
Recent studies have pointed to changes in tissue mechanics as a contributory element to the development of malignancies. Increased tissue rigidity is associated with the unfolding of the Type III domains of fibronectin within the extracellular matrix. The consequences of this unfolding on cellular functions within the lung are not well understood. In the present study, we evaluated the effect of a peptide representing a partially unfolded intermediate of the first Type III repeat of fibronectin (FnIII-1c) on inflammatory gene expression in adult human lung fibroblast cells. FnIII-1c induced expression of cytokines, CXCL1-3, IL-8 and TNF-α, by lung fibroblast cells. The increase in IL-8 expression was dependent on Toll-like receptor 2 and NFκB. Immunohistochemistry of tissue arrays representing squamous cell carcinoma of the lung revealed extensive stromal staining for IL-8 and fibronectin fibrils which were co-aligned with myofibroblasts. These data suggest a model in which unfolding of FnIII domains secondary to myofibroblast-generated tension may induce the release of cytokines by stromal fibroblasts present within the lung tumor.
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影响因子：
5.2
作者：
Jones, Carla P.;Pitchford, Simon C.;Lloyd, Clare M.;Rankin, Sara M.
通讯作者：
Rankin, Sara M.
DOI：
10.1073/pnas.98.2.620
发表时间：
2001-01-16
影响因子：
11.1
作者：
Yi, M;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
3.4
作者：
Antia, Meher;Baneyx, Gretchen;Vogel, Viola
通讯作者：
Vogel, Viola
影响因子：
38.9
作者：
Lorne, Emmanuel;Dupont, Herve;Abraham, Edward
通讯作者：
Abraham, Edward
影响因子：
4.8
作者：
Gui, Liqiong;Wojciechowski, Katherine;Hocking, Denise C.
通讯作者：
Hocking, Denise C.